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Western Blot (WB)
(Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human CD14 Polyclonal Antibody | anti-CD14 antibody
CD14, CT (CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form) (FITC)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
CD14; Polyclonal Antibody; CT (CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; urinary form; membrane-bound form) (FITC); membrane-bound form; anti-CD14 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-CD14 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300~329 amino acids from the C-terminal region of human CD14.
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(CD14 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).)
Western Blot (WB)
(CD14 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CD14 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CD14 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CD14 antibody
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Product Categories/Family for anti-CD14 antibody
References
Donati, M., J. Periodontol. 79 (3), 517-524 (2008). Yuan, F.F., Immunol. Cell Biol. 86 (3), 268-270 (2008). Setoguchi, M., Biochim. Biophys. Acta 1008 (2), 213-222 (1989) Goyert, S.M., Science 239 (4839), 497-500 (1988).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53kDa
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
Protein Family
UniProt Gene Name
CD14
UniProt Entry Name
CD14_HUMAN
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Product Notes
The CD14 cd14 (Catalog #AAA6282776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD14, CT (CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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