Rabbit anti-Human CD14 Polyclonal Antibody | anti-CD14 antibody

CD14 Antibody (C-term)

Cat. Num. AAA9214910

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CD14; Polyclonal Antibody; CD14 Antibody (C-term); Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; urinary form; membrane-bound form; anti-CD14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-322 amino acids from the C-terminal region of human CD14.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 292-322
• Sequence Length: 375

• Applicable Applications for anti-CD14 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(CD14 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CD14 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CD14 antibody

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.

Product Categories/Family for anti-CD14 antibody

Immunology; Signal Transduction

References

Donati,M., J. Periodontol. 79 (3), 517-524 (2008)
Yuan,F.F., Immunol. Cell Biol. 86 (3), 268-270 (2008)
Setoguchi,M., Biochim. Biophys. Acta 1008 (2), 213-222 (1989)
Goyert,S.M., Science 239 (4839), 497-500 (1988)

NCBI and Uniprot Product Information

• NCBI GI #: 4557417
• NCBI GeneID: 929
• NCBI Accession #: NP_000582.1; NP_001035110.1; NP_001167575.1; NP_001167576.1
• NCBI GenBank Nucleotide #: NM_000591.3
• UniProt Accession #: P08571; Q53XT5; Q96FR6; Q96L99; Q9UNS3
• Molecular Weight: 40076
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_HUMAN

NCBI Description

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Uniprot Description

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; endosome membrane; lipid raft

Molecular Function: peptidoglycan receptor activity; protein binding; opsonin receptor activity; lipopolysaccharide binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; apoptosis; MyD88-independent toll-like receptor signaling pathway; positive regulation of endocytosis; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; phagocytosis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to magnesium ion; response to ethanol; cell surface receptor linked signal transduction; response to heat; toll-like receptor signaling pathway; innate immune response; response to electrical stimulus; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of cytokine secretion