Western Blot (WB)
(Western blot analysis of SEMA3A Antibody (Center) in CEM cell line lysates (35ug/lane). SEMA3A (arrow) was detected using the purified Pab.)
Rabbit anti-Human SEMA3A Polyclonal Antibody | anti-SEMA3A antibody
SEMA3A, ID (Semaphorin-3A, Semaphorin III, Sema III, SEMA3A, SEMAD)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Western Blot (WB)
(Western blot analysis of SEMA3A Antibody (Center) in CEM cell line lysates (35ug/lane). SEMA3A (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of SEMA3A Antibody (Center) in CEM cell line lysates (35ug/lane). SEMA3A (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SEMA3A antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SEMA3A antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq]
Uniprot Description
SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7p12.1
Cellular Component: membrane; axon; dendrite; extracellular region
Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding
Biological Process: trigeminal nerve structural organization; facial nerve structural organization; axon guidance; regulation of heart rate; apoptosis; olfactory bulb development; dendrite morphogenesis; neuron migration; axonal fasciculation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development
Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia
Research Articles on SEMA3A
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Product Notes
The SEMA3A sema3a (Catalog #AAA628050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEMA3A, ID (Semaphorin-3A, Semaphorin III, Sema III, SEMA3A, SEMAD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA3A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the SEMA3A sema3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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