Western Blot (WB)
(Western blot analysis of C21orf29 Antibody (Center) in MDA-MB231, K562 cell line lysates (35ug/lane). C21orf29 (arrow) was detected using the purified Pab.)
Rabbit anti-Human C21orf29 Polyclonal Antibody | anti-C21orf29 antibody
C21orf29, ID (TSPEAR, C21orf29, Protein TSPEAR, Thrombospondin-type laminin G domain and EAR repeat-containing protein) (FITC)
Gene Names
TSPEAR; DFNB98; TSP-EAR; C21orf29
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
C21orf29; Polyclonal Antibody; ID (TSPEAR; Protein TSPEAR; Thrombospondin-type laminin G domain and EAR repeat-containing protein) (FITC); TSPEAR; Thrombospondin-type laminin G domain and EAR repeat-containing protein; anti-C21orf29 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-C21orf29 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
C21orf29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299~329 amino acids from the Center region of human C21orf29.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of C21orf29 Antibody (Center) in MDA-MB231, K562 cell line lysates (35ug/lane). C21orf29 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of C21orf29 Antibody (Center) in MDA-MB231, K562 cell line lysates (35ug/lane). C21orf29 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with C21orf29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with C21orf29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(C21orf29 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(C21orf29 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-C21orf29 antibody
References
Scheel, H., et.al., Hum. Mol. Genet. 11 (15), 1757-1762 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,924 Da
NCBI Official Full Name
thrombospondin-type laminin G domain and EAR repeat-containing protein isoform 2
NCBI Official Synonym Full Names
thrombospondin-type laminin G domain and EAR repeats
NCBI Official Symbol
TSPEAR
NCBI Official Synonym Symbols
DFNB98; TSP-EAR; C21orf29
NCBI Protein Information
thrombospondin-type laminin G domain and EAR repeat-containing protein; thrombospondin-type laminin G domain and EAR repeats-containing protein
UniProt Protein Name
Thrombospondin-type laminin G domain and EAR repeat-containing protein
UniProt Gene Name
TSPEAR
UniProt Synonym Gene Names
C21orf29; TSP-EAR
UniProt Entry Name
TSEAR_HUMAN
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Product Notes
The C21orf29 tspear (Catalog #AAA6279817) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C21orf29, ID (TSPEAR, C21orf29, Protein TSPEAR, Thrombospondin-type laminin G domain and EAR repeat-containing protein) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C21orf29 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the C21orf29 tspear for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C21orf29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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