Rabbit anti-Human BTRC Polyclonal Antibody | anti-BTRC antibody

BTRC, NT (BTRC, BTRCP, FBW1A, FBXW1A, F-box/WD repeat-containing protein 1A, E3RSIkappaB, Epididymis tissue protein Li 2a, F-box and WD repeats protein beta-TrCP, pIkappaBalpha-E3 receptor subunit) (Biotin)

Cat. Num. AAA6279310

• Gene Names: BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BTRC; Polyclonal Antibody; NT (BTRC; BTRCP; FBW1A; FBXW1A; F-box/WD repeat-containing protein 1A; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; pIkappaBalpha-E3 receptor subunit) (Biotin); pIkappaBalpha-E3 receptor subunit; anti-BTRC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BTRC antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BTRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-60 amino acids from the N-terminal region of human BTRC.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BTRC Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the BTRC antibody detected the BTRC protein (arrow).)

Immunohistochemistry (IHC)

(BTRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of BTRC Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-BTRC antibody

Antibodies; WD Repeat Proteins

References

Popov, N., et al. Nat. Cell Biol. 12(10):973-981(2010). Inuzuka, H., et al. Cancer Cell 18(2):147-159(2010). Guderian, G., et al. J. Cell. Sci. 123 (PT 13), 2163-2169 (2010): Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Tsai, W.B., et al. PLoS ONE 5 (7), E11171 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 16117783
• NCBI GeneID: 8945
• NCBI Accession #: NP_378663
• NCBI GenBank Nucleotide #: NM_033637.3
• UniProt Accession #: Q9Y297; Q5W141; Q5W142; Q9Y213; B5MD49
• Molecular Weight: 65,049 Da
• NCBI Official Full Name: F-box/WD repeat-containing protein 1A isoform 1
• NCBI Official Synonym Full Names: beta-transducin repeat containing E3 ubiquitin protein ligase
• NCBI Official Symbol: BTRC
• NCBI Official Synonym Symbols: FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
• NCBI Protein Information: F-box/WD repeat-containing protein 1A
• UniProt Protein Name: F-box/WD repeat-containing protein 1A
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: BTRC
• UniProt Synonym Gene Names: BTRCP; FBW1A; FBXW1A
• UniProt Entry Name: FBW1A_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

BTRC: a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Regulates the stability of CTNNB1 and participates in Wnt signaling. Interacts directly with SKP1 in the SCF complex. Interacts specifically with phosphorylated CTNNB1 and NFKBIA, ubiquitination substrates. Two alternatively spliced isoforms have been described.

Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: nucleoplasm; SCF ubiquitin ligase complex; cytosol

Molecular Function: protein dimerization activity; protein phosphorylated amino acid binding; protein binding; ubiquitin-protein ligase activity; beta-catenin binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; viral reproduction; regulation of cell cycle; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; protein ubiquitination; positive regulation of circadian rhythm; protein amino acid dephosphorylation; signal transduction; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; G2/M transition of mitotic cell cycle; regulation of I-kappaB kinase/NF-kappaB cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; protein destabilization; negative regulation of transcription factor activity; mammary gland epithelial cell proliferation; regulation of circadian rhythm; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of smoothened signaling pathway; mitotic cell cycle; negative regulation of transcription, DNA-dependent