Rabbit anti-Human, Mouse BMP7 Polyclonal Antibody | anti-BMP7 antibody

BMP7, CT (BMP7, OP1, Bone morphogenetic protein 7, Osteogenic protein 1, INN=Eptotermin alfa) (AP)

Cat. Num. AAA6278593

• Gene Names: BMP7; OP-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: BMP7; Polyclonal Antibody; CT (BMP7; OP1; Bone morphogenetic protein 7; Osteogenic protein 1; INN=Eptotermin alfa) (AP); INN=Eptotermin alfa; anti-BMP7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BMP7. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-BMP7 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 280-309 in the C-terminal region of human BMP7, UniProt Accession #NP_001710.1; P18075.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in 293 cell lysates (2ug/lane) usingMBS6010462, either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BMP7 gene.)

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS6010462. This demonstrates that MBS6010462 detected the BMP7 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6010462 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6010462 for immunohistochemistry.)

Product Categories/Family for anti-BMP7 antibody

Antibodies; Growth Factors; Cytokines

References

1. Hwang, C.J., et al. J Neurosurg Spine 13(4):484-493(2010). 2. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). 3. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 4. Jonigk, D., et al. Virchows Arch. 457(3):369-380(2010). 5. Pegorier, S., et al. Respir. Res. 11, 85 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4502427
• NCBI GeneID: 655
• NCBI Accession #: NP_001710.1
• NCBI GenBank Nucleotide #: NM_001719.2
• UniProt Accession #: P18075; Q9H512; Q9NTQ7
• Molecular Weight: 49,313 Da
• NCBI Official Full Name: bone morphogenetic protein 7
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: BMP7
• NCBI Official Synonym Symbols: OP-1
• NCBI Protein Information: bone morphogenetic protein 7; osteogenic protein 1
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP7
• UniProt Synonym Gene Names: OP1; BMP-7; OP-1
• UniProt Entry Name: BMP7_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: negative regulation of MAP kinase activity; axon guidance; response to peptide hormone stimulus; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; embryonic pattern specification; response to estradiol stimulus; negative regulation of cell cycle; BMP signaling pathway; regulation of apoptosis; response to vitamin D; epithelial cell differentiation; ureteric bud development; dendrite development; mesonephros development; skeletal development; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; mesenchymal cell differentiation; branching morphogenesis of a tube; mesoderm formation; inhibition of NF-kappaB transcription factor; cartilage development; negative regulation of mitosis; epithelial to mesenchymal transition; negative regulation of phosphorylation; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; metanephros development; neurite morphogenesis; growth