Rabbit anti-Human ATP5B Polyclonal Antibody | anti-Atp5b antibody

ATP5B, ID (ATP Synthase Subunit beta, Mitochondrial, ATPSB, ATPMB)

Cat. Num. AAA627853

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ATP5B; Polyclonal Antibody; ID (ATP Synthase Subunit beta; Mitochondrial; ATPSB; ATPMB); Anti -ATP5B; anti-Atp5b antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATP5B.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Atp5b antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:100-1:500; Flow Cytometry: 1:10-1:50; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide corresponding to aa142-171 from the center region of human ATP5B conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP5B Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ATP5B (arrow) using rabbit polyclonal ATP5B Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2) .)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Atp5b antibody

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.

Product Categories/Family for anti-Atp5b antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 23272966
• NCBI GeneID: 11947
• UniProt Accession #: P56480; Q0QEP4; Q3TFD7; Q3TIP9; Q3TK44; Q3TWD5; Q3TX28; Q3U6U4; Q3U774; Q3UB69; Q3UF69; Q8CI65
• Molecular Weight: 56,300 Da
• NCBI Official Full Name: Atp5b protein
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit
• NCBI Official Symbol: Atp5b
• NCBI Protein Information: ATP synthase subunit beta, mitochondrial; OTTMUSP00000027963; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit
• UniProt Protein Name: ATP synthase subunit beta, mitochondrial
• UniProt Gene Name: Atp5b
• UniProt Entry Name: ATPB_MOUSE

Uniprot Description

ATP5B: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase alpha/beta chains family.

Protein type: Mitochondrial; Hydrolase; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Cellular Component: cell surface; membrane; mitochondrion; proton-transporting ATP synthase complex, catalytic core F(1); mitochondrial inner membrane; mitochondrial membrane; plasma membrane; nucleus; myelin sheath; mitochondrial proton-transporting ATP synthase complex

Molecular Function: lipoprotein receptor activity; hydrolase activity; MHC class I protein binding; ATPase activity; nucleotide binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; calcium ion binding; hydrogen ion transporting ATPase activity, rotational mechanism; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; ATP binding

Biological Process: receptor-mediated endocytosis; substrate-bound cell migration, cell release from substrate; ATP synthesis coupled proton transport; ADP biosynthetic process; ATP metabolic process; osteoblast differentiation; proton transport; transport; ATP biosynthetic process; ATP hydrolysis coupled proton transport; regulation of intracellular pH; ion transport; angiogenesis; lipid metabolic process