Rabbit anti-Human, Mouse BHLH3 Polyclonal Antibody | anti-BHLH3 antibody

BHLH3, NT (BHLHE41, BHLHB3, DEC2, SHARP1, Class E basic helix-loop-helix protein 41, Class B basic helix-loop-helix protein 3, Differentially expressed in chondrocytes protein 2, Enhancer-of-split and hairy-related protein 1) (Azide free) (HRP)

Cat. Num. AAA6278333

• Gene Names: BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BHLH3; Polyclonal Antibody; NT (BHLHE41; BHLHB3; DEC2; SHARP1; Class E basic helix-loop-helix protein 41; Class B basic helix-loop-helix protein 3; Differentially expressed in chondrocytes protein 2; Enhancer-of-split and hairy-related protein 1) (Azide free) (HRP); BHLHE41; Enhancer-of-split and hairy-related protein 1; anti-BHLH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BHLH3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human BHLH3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BHLH3 Antibody (N-term) western blot analysis in RD cell line lysates (35ug/lane).This demonstrates the BHE41 antibody detected the BHE41 protein (arrow).)

Flow Cytometry (FC/FACS)

(BHLH3 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly. )

Related Product Information for anti-BHLH3 antibody

BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.

Product Categories/Family for anti-BHLH3 antibody

Antibodies; Transcription Factors

References

Honma, S., et al. Nature 419(6909):841-844(2002). Garriga-Canut, M., et al. J. Biol. Chem. 276(18):14821-14828(2001). Fujimoto, K., et al. Biochem. Biophys. Res. Commun. 280(1):164-171(2001). Grottke, C., et al. Int. J. Cancer 88(4):535-546(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 13540521
• NCBI GeneID: 79365
• NCBI Accession #: NP_110389
• NCBI GenBank Nucleotide #: NM_030762
• UniProt Accession #: Q9C0J9
• Molecular Weight: 50kDa
• NCBI Official Full Name: class E basic helix-loop-helix protein 41
• NCBI Official Synonym Full Names: basic helix-loop-helix family member e41
• NCBI Official Symbol: BHLHE41
• NCBI Official Synonym Symbols: DEC2; hDEC2; BHLHB3; SHARP1
• NCBI Protein Information: class E basic helix-loop-helix protein 41
• UniProt Protein Name: Class E basic helix-loop-helix protein 41
• Protein Family: Transcription factor
• UniProt Gene Name: BHLHE41
• UniProt Synonym Gene Names: BHLHB3; DEC2; SHARP1; bHLHe41; bHLHb3; hDEC2; SHARP-1
• UniProt Entry Name: BHE41_HUMAN

NCBI Description

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]

Uniprot Description

BHLHB3: May be a transcriptional repressor that represses both basal and activated transcription. Homodimerize.

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; MRF binding; protein binding; protein homodimerization activity; protein heterodimerization activity; histone deacetylase binding; bHLH transcription factor binding; transcription corepressor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; cell proliferation; organ morphogenesis; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; cell differentiation

Disease: Short Sleeper