Rabbit anti-Human BEST3 Polyclonal Antibody | anti-BEST3 antibody

BEST3, NT (BEST3, VMD2L3, Bestrophin-3, Vitelliform macular dystrophy 2-like protein 3) (Azide free) (HRP)

Cat. Num. AAA6278267

• Gene Names: BEST3; VMD2L3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BEST3; Polyclonal Antibody; NT (BEST3; VMD2L3; Bestrophin-3; Vitelliform macular dystrophy 2-like protein 3) (Azide free) (HRP); Vitelliform macular dystrophy 2-like protein 3; anti-BEST3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BEST3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BEST3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from the N-terminal region of human BEST3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BEST3 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the BEST3 antibody detected the BEST3 protein (arrow).)

Related Product Information for anti-BEST3 antibody

BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).

Product Categories/Family for anti-BEST3 antibody

Antibodies; Disease Markers

References

Marsey, L.L., et al. J. Physiol. (Lond.) 587 (PT 10), 2211-2224 (2009): Qu, Z.Q., et al. J. Biol. Chem. 282(24):17460-17467(2007). Tsunenari, T., et al. J. Biol. Chem. 278(42):41114-41125(2003). Stohr, H., et al. Eur. J. Hum. Genet. 10(4):281-284(2002). Marquardt, A., et al. Hum. Mol. Genet. 7(9):1517-1525(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 115527104
• NCBI GeneID: 144453
• NCBI Accession #: NP_116124.2
• NCBI GenBank Nucleotide #: NM_032735.2
• UniProt Accession #: Q8N1M1; Q53YQ7; Q8N356; Q8NFT9; Q9BR80; B5MDI8; F8VVZ2
• Molecular Weight: 63,378 Da
• NCBI Official Full Name: bestrophin-3 isoform 1
• NCBI Official Synonym Full Names: bestrophin 3
• NCBI Official Symbol: BEST3
• NCBI Official Synonym Symbols: VMD2L3
• NCBI Protein Information: bestrophin-3; vitelliform macular dystrophy 2-like 3; vitelliform macular dystrophy 2-like protein 3
• UniProt Protein Name: Bestrophin-3
• Protein Family: Bestrophin
• UniProt Gene Name: BEST3
• UniProt Synonym Gene Names: VMD2L3
• UniProt Entry Name: BEST3_HUMAN