Rabbit anti-Human BCAT2 Polyclonal Antibody | anti-BCAT2 antibody

BCAT2, CT (BCAT2, BCATM, BCT2, ECA40, Branched-chain-amino-acid aminotransferase, mitochondrial, Placental protein 18) (HRP)

Cat. Num. AAA6277816

• Gene Names: BCAT2; BCAM; BCT2; PP18; BCATM
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: BCAT2; Polyclonal Antibody; CT (BCAT2; BCATM; BCT2; ECA40; Branched-chain-amino-acid aminotransferase; mitochondrial; Placental protein 18) (HRP); Placental protein 18; anti-BCAT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BCAT2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: BCAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the C-terminal region of human BCAT2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of BCAT2 (arrow) using rabbit polyclonal BCAT2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCAT2 gene.)

Immunohistochemistry (IHC)

(BCAT2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BCAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-BCAT2 antibody

Antibodies; Enzymes; Aminotransferase

References

Coles, S.J., et al. Biochemistry 48(3):645-656(2009). Yennawar, N.H., et al. J. Biol. Chem. 281(51):39660-39671(2006). Goto, M., et al. J. Biol. Chem. 280(44):37246-37256(2005). Conway, M.E., et al. Biochemistry 43(23):7356-7364(2004). Yennawar, N., et al. Acta Crystallogr. D Biol. Crystallogr. 57 (PT 4), 506-515 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 258614015
• NCBI GeneID: 587
• NCBI Accession #: NP_001158245.1
• NCBI GenBank Nucleotide #: NM_001164773.1
• UniProt Accession #: O15382; O00269; Q96KG1; Q9BTB6; Q9BUU6; B2RB87
• Molecular Weight: 33,777 Da
• NCBI Official Full Name: branched-chain-amino-acid aminotransferase, mitochondrial isoform b
• NCBI Official Synonym Full Names: branched chain amino-acid transaminase 2, mitochondrial
• NCBI Official Symbol: BCAT2
• NCBI Official Synonym Symbols: BCAM; BCT2; PP18; BCATM
• NCBI Protein Information: branched-chain-amino-acid aminotransferase, mitochondrial; branched chain aminotransferase 2, mitochondrial; placental protein 18
• UniProt Protein Name: Branched-chain-amino-acid aminotransferase, mitochondrial
• UniProt Gene Name: BCAT2
• UniProt Synonym Gene Names: BCATM; BCT2; ECA40; BCAT(m); PP18
• UniProt Entry Name: BCAT2_HUMAN

NCBI Description

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; EC 2.6.1.42; Transferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: branched-chain-amino-acid transaminase activity

Biological Process: valine metabolic process; branched chain family amino acid catabolic process; leucine metabolic process; isoleucine catabolic process; branched chain family amino acid biosynthetic process