Rabbit anti-Human, Mouse B4GALT5 Polyclonal Antibody | anti-B4GALT5 antibody

B4GALT5, CT (B4GALT5, Beta-1,4-galactosyltransferase 5, Beta-1,4-GalT II, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5) (APC)

Cat. Num. AAA6277229

• Gene Names: B4GALT5; gt-V; B4Gal-T5; beta4Gal-T5; beta4GalT-V; BETA4-GALT-IV
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: B4GALT5; Polyclonal Antibody; CT (B4GALT5; Beta-1; 4-galactosyltransferase 5; 4-GalT II; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1; 4-galactosyltransferase 5) (APC); anti-B4GALT5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-B4GALT5 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: B4GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the C-terminal region of human B4GALT5.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(B4GALT5 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the B4GALT5 antibody detected B4GALT5 protein (arrow).)

Flow Cytometry (FC/FACS)

(B4GALT5 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-B4GALT5 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Kitayama, K., et al. J. Biol. Chem. 282(41):30085-30096(2007). Sato, T., et al. J. Biol. Chem. 282(38):27702-27712(2007). Jiang, J., et al. J. Biol. Chem. 281(14):9482-9489(2006). Sato, T., et al. J. Biol. Chem. 279(38):39574-39583(2004). Xu, S., et al. J. Exp. Clin. Cancer Res. 21(3):409-414(2002). Deloukas, P., et al. Nature 414(6866):865-871(2001). Amado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999). Lo, N.W., et al. Glycobiology 8(5):517-526(1998). Sato, T., et al. Biochem. Biophys. Res. Commun. 244(3):637-641(1998). Sato, T., et al. Proc. Natl. Acad. Sci. U.S.A. 95(2):472-477(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4757828
• NCBI GeneID: 9334
• NCBI Accession #: NP_004767.1
• NCBI GenBank Nucleotide #: NM_004776.3
• UniProt Accession #: O43286; Q2M394; Q9UJQ8; E1P625
• Molecular Weight: 45,119 Da
• NCBI Official Full Name: beta-1,4-galactosyltransferase 5
• NCBI Official Synonym Full Names: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
• NCBI Official Symbol: B4GALT5
• NCBI Official Synonym Symbols: gt-V; B4Gal-T5; beta4Gal-T5; beta4GalT-V; BETA4-GALT-IV
• NCBI Protein Information: beta-1,4-galactosyltransferase 5; beta4-GalT IV; beta-1,4-GalT II; beta-1,4-GalT IV; beta-1,4-GalTase 5; beta-1.4-galactosyltransferase V; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide
• UniProt Protein Name: Beta-1,4-galactosyltransferase 5
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT5
• UniProt Synonym Gene Names: Beta-1,4-GalTase 5; Beta4Gal-T5; b4Gal-T5
• UniProt Entry Name: B4GT5_HUMAN

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. [provided by RefSeq, Jul 2008]

Uniprot Description

B4GALT5: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Belongs to the glycosyltransferase 7 family.

Protein type: Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.-; Transferase

Chromosomal Location of Human Ortholog: 20q13.1-q13.2

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: metal ion binding; galactosyltransferase activity

Biological Process: keratan sulfate metabolic process; protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification