Western Blot (WB)
(B3GNT1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the B3GNT1 antibody detected the B3GNT1 protein (arrow).)
Rabbit anti-Human B3GNT1 Polyclonal Antibody | anti-B3GNT1 antibody
B3GNT1, CT (B3GNT1, B3GNT6, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, Poly-N-acetyllactosamine extension enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1) (AP)
Gene Names
B3GNT1; iGAT; iGNT; B3GNT6; B3GN-T1; MDDGA13; BETA3GNTI
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
B3GNT1; Polyclonal Antibody; CT (B3GNT1; B3GNT6; N-acetyllactosaminide beta-1; 3-N-acetylglucosaminyltransferase; I-beta-1; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 1) (AP); 3-N-acetylglucosaminyltransferase 1; anti-B3GNT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-B3GNT1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
B3GNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-394 amino acids from the C-terminal region of human B3GNT1.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(B3GNT1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the B3GNT1 antibody detected the B3GNT1 protein (arrow).)
Western Blot (WB)
(B3GNT1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the B3GNT1 antibody detected the B3GNT1 protein (arrow).)
Immunohistochemistry (IHC)
(B3GNT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of B3GNT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(B3GNT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of B3GNT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-B3GNT1 antibody
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen.
Product Categories/Family for anti-B3GNT1 antibody
References
Bao, X., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):12109-12114(2009). Lee, P.L., et al. Glycobiology 19(6):655-664(2009). Sasaki, K., et al. Proc. Natl. Acad. Sci. U.S.A. 94(26):14294-14299(1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,119 Da
NCBI Official Full Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
NCBI Official Symbol
B3GNT1
NCBI Official Synonym Symbols
iGAT; iGNT; B3GNT6; B3GN-T1; MDDGA13; BETA3GNTI
NCBI Protein Information
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; poly-N-acetyllactosamine extension enzyme; i-beta-1,3-N-acetylglucosaminyltransferase; beta-1,3-N-acetylglucosaminyltransferase bGnT-6; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransfe
UniProt Protein Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
UniProt Gene Name
B3GNT1
UniProt Synonym Gene Names
B3GNT6; iGnT
UniProt Entry Name
B3GN1_HUMAN
Similar Products
Product Notes
The B3GNT1 b3gnt1 (Catalog #AAA6277129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B3GNT1, CT (B3GNT1, B3GNT6, N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, Poly-N-acetyllactosamine extension enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B3GNT1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the B3GNT1 b3gnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GNT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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