Rabbit Aurora-B Polyclonal Antibody | anti-AURKB antibody

Aurora-B, NT (AURKB, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora kinase B, Aurora 1, Aurora-and IPL1-like midbody-associated protein 1, Aurora/IPL1-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Seri

Cat. Num. AAA6276889

• Gene Names: AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Aurora-B; Polyclonal Antibody; NT (AURKB; AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; Aurora kinase B; Aurora 1; Aurora-and IPL1-like midbody-associated protein 1; Aurora/IPL1-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Seri; AURKB; Serine/threonine-protein kinase aurora-B; anti-AURKB antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-AURKB antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Aurora-B (ARK/STK12) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~43 amino acids from the N-terminal region of human Aurora-B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-STK12 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). STK12 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(The STK12 polyclonal antibody is used in Western blot to detect STK12 in mouse brain tissue lysate.)

Western Blot (WB)

(Western blot analysis of AURKB (arrow) using rabbit polyclonal Aurora-B (ARK/STK12) Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-AURKB antibody

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. STK12 (Aurora kinase B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes

Product Categories/Family for anti-AURKB antibody

Antibodies; Protein Kinases

References

Kimura, M., et al., Biochem. Biophys. Res. Commun. 316(3):930-936 (2004). Yasui, Y., et al., J. Biol. Chem. 279(13):12997-13003 (2004). Lampson, M.A., et al., Nat. Cell Biol. 6(3):232-237 (2004). Wheatley, S.P., et al., J. Biol. Chem. 279(7):5655-5660 (2004). Honda, R., et al., Mol. Biol. Cell 14(8):3325-3341 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 83776600
• NCBI GeneID: 9212
• NCBI Accession #: NP_004208.2
• NCBI GenBank Nucleotide #: NM_004217.3
• UniProt Accession #: Q96GD4; O14630; O60446; O95083; Q96DV5; Q9UQ46; C7G533; C7G534; C7G535; D3DTR4
• Molecular Weight: 39,311 Da
• NCBI Official Full Name: aurora kinase B isoform 1
• NCBI Official Synonym Full Names: aurora kinase B
• NCBI Official Symbol: AURKB
• NCBI Official Synonym Symbols: AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
• NCBI Protein Information: aurora kinase B; ARK-2; STK-1; aurora 1; aurora-1; aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; aurora-related kinase 2; serine/threonine kinase 12; aurora/IPL1-related kinase 2; serine/threonine-protein kinase 5; serine/threonine-protein kinase 12
• UniProt Protein Name: Aurora kinase B
• Protein Family: Aurora kinase
• UniProt Gene Name: AURKB
• UniProt Synonym Gene Names: AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2
• UniProt Entry Name: AURKB_HUMAN

NCBI Description

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

AurB: a member of the AUR family of kinases. May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Expressed during S and G2/M phase and expression is upregulated in cancer cells during M phase. Localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis. Colocalizes with gamma tubulin in the mid-body. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Overexpressed in colorectal and other cancer cell lines and thought to cause aneuploidy via histone phosphorylation.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; AUR family

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: spindle microtubule; spindle; spindle midzone; spindle pole centrosome; midbody; nucleus; cytosol; condensed nuclear chromosome, pericentric region; chromocenter

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; ATP binding

Biological Process: positive regulation of cytokinesis; spindle stabilization; spindle checkpoint; spindle midzone assembly involved in mitosis; protein amino acid autophosphorylation; regulation of chromosome segregation; negative regulation of transcription from RNA polymerase II promoter; attachment of spindle microtubules to kinetochore; protein amino acid phosphorylation; negative regulation of cytokinesis; cell proliferation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; histone modification; abscission; mitotic cell cycle; negative regulation of protein binding; negative regulation of B cell apoptosis; aging