Rabbit anti-Human ATP1B2 Polyclonal Antibody | anti-ATP1B2 antibody

ATP1B2, ID (ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Sodium/potassium-dependent ATPase subunit beta-2) (Azide free) (HRP)

Cat. Num. AAA6276429

• Gene Names: ATP1B2; AMOG
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP1B2; Polyclonal Antibody; ID (ATP1B2; Sodium/potassium-transporting ATPase subunit beta-2; Sodium/potassium-dependent ATPase subunit beta-2) (Azide free) (HRP); Sodium/potassium-dependent ATPase subunit beta-2; anti-ATP1B2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ATP1B2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATP1B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121~150 amino acids from the Center region of human ATP1B2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATP1B2 Antibody (Center) in K562 cell line lysates (35ug/lane). ATP1B2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ATP1B2 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ATP1B2 antibody

The protein belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

Product Categories/Family for anti-ATP1B2 antibody

Antibodies; Ion Channel

References

Guey, L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010). Tokhtaeva, E., et.al., Biochemistry 48 (48), 11421-11431 (2009). Hosgood, H.D. et.al., Respir Med 103 (12), 1866-1870 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 49574491
• NCBI GeneID: 482
• NCBI Accession #: NP_001669.3
• NCBI GenBank Nucleotide #: NM_001678.4
• UniProt Accession #: P14415; O60444; A0AV17; A8K278; D3DTQ2
• Molecular Weight: 33,367 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-2 isoform 1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 2 polypeptide
• NCBI Official Symbol: ATP1B2
• NCBI Official Synonym Symbols: AMOG
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-2; Na, K-ATPase beta-2 polypeptide; adhesion molecule in glia; adhesion molecule on glia; sodium pump subunit beta-2; sodium-potassium ATPase subunit beta 2 (non-catalytic); sodium/potassium-dependent ATPa
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-2
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1B2
• UniProt Synonym Gene Names: AMOG
• UniProt Entry Name: AT1B2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

ATP1B2: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Belongs to the X(+)/potassium ATPases subunit beta family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; sodium:potassium-exchanging ATPase complex

Molecular Function: ATPase activator activity; protein binding; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: cellular sodium ion homeostasis; protein stabilization; potassium ion import; transport; positive regulation of ATPase activity; blood coagulation; cell adhesion; leukocyte migration; cellular potassium ion homeostasis; transmembrane transport