Sequence
Complement Component 1, Q Subcomponent B (C1qB) Active Protein | C1qB active protein
Active Complement Component 1, Q Subcomponent B (C1qB)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity Data
(The complement component 1q (or simply C1q) is a protein complex involved in the complement system, which is part of the innate immune system. C1q together with C1r and C1s form the C1 complex. Antibodies of the adaptive immune system can bind antigen, forming an antigen-antibody complex. When C1q binds antigen-antibody complexes, the C1 complex becomes activated. Activation of the C1 complex intitiates the classical complement pathway of the complement system. The antibodies IgM and all IgG subclasses except IgG4 are able to initiate the complement system. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Complement Component 1, Q Subcomponent B (C1qB) is six B-chains of C1q. Besides, Profilin 1 (PFN1) has been identified as an interactor of C1qB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human C1qB and recombinant human PFN1. Briefly, C1qB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C1qB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of C1qB and PFN1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure 1. The binding activity of C1qB with PFN1.)
Activity Data
(The complement component 1q (or simply C1q) is a protein complex involved in the complement system, which is part of the innate immune system. C1q together with C1r and C1s form the C1 complex. Antibodies of the adaptive immune system can bind antigen, forming an antigen-antibody complex. When C1q binds antigen-antibody complexes, the C1 complex becomes activated. Activation of the C1 complex intitiates the classical complement pathway of the complement system. The antibodies IgM and all IgG subclasses except IgG4 are able to initiate the complement system. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Complement Component 1, Q Subcomponent B (C1qB) is six B-chains of C1q. Besides, Profilin 1 (PFN1) has been identified as an interactor of C1qB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human C1qB and recombinant human PFN1. Briefly, C1qB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C1qB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of C1qB and PFN1 was shown in Figure 1, and this effect was in a dose dependent manner.Figure 1. The binding activity of C1qB with PFN1.)
SDS-Page
(Figure 2. SDS-PAGESample: Active recombinant C1qB, Human)
SDS-Page
(Figure 2. SDS-PAGESample: Active recombinant C1qB, Human)
Western Blot (WB)
(Figure 3. Western BlotSample: Recombinant C1qB, Human;Antibody: Rabbit Anti-Human C1qB Ab ( MBS2112297 ))
Western Blot (WB)
(Figure 3. Western BlotSample: Recombinant C1qB, Human;Antibody: Rabbit Anti-Human C1qB Ab ( MBS2112297 ))
NCBI and Uniprot Product Information
Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
This gene encodes the B-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. [provided by RefSeq, Dec 2016]
Research Articles on C1qB
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Product Notes
The C1qB (Catalog #AAA2105181) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Complement Component 1, Q Subcomponent B (C1qB) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Complement Component 1, Q Subcomponent B (C1qB) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the C1qB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement Component 1, Q Subcomponent B (C1qB), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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