Rabbit ATL3 Polyclonal Antibody | anti-ATL3 antibody

ATL3, ID (ATL3, Atlastin-3) (FITC)

Cat. Num. AAA6276318

• Gene Names: ATL3; DKFZp564J0863
• Reactivity: Human, Hamster, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATL3; Polyclonal Antibody; ID (ATL3; Atlastin-3) (FITC); Atlastin-3; anti-ATL3 antibody

• Host: Rabbit
• Reactivity: Human, Hamster, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ATL3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 230-260 from the Central region of human ATL3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATL3 Antibody (Center) in mouse liver tissue lysates (35ug/lane). ATL3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ATL3 Antibody (Center) in CHO cell line lysates (35ug/lane). ATL3 (arrow) was detected using the purified Pab.)

Related Product Information for anti-ATL3 antibody

ATL3 is GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. ATL3 is function in endoplasmic reticulum tubular network biogenesis.

Product Categories/Family for anti-ATL3 antibody

Antibodies; Proteins

References

Hu, J., et al. Cell 138(3):549-561(2009). Rismanchi, N., et al. Hum. Mol. Genet. 17(11):1591-1604(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 45827806
• NCBI GeneID: 25923
• NCBI Accession #: NP_056274.3
• NCBI GenBank Nucleotide #: NM_015459.3
• UniProt Accession #: Q6DD88; Q8N7W5; Q9H8Q5; Q9UFL1
• Molecular Weight: 60,542 Da
• NCBI Official Full Name: atlastin-3
• NCBI Official Synonym Full Names: atlastin GTPase 3
• NCBI Official Symbol: ATL3
• NCBI Official Synonym Symbols: DKFZp564J0863
• NCBI Protein Information: atlastin-3; atlastin 3; OTTHUMP00000237071; OTTHUMP00000237072
• UniProt Protein Name: Atlastin-3
• Protein Family: Atlastin
• UniProt Gene Name: ATL3
• UniProt Entry Name: ATLA3_HUMAN

NCBI Description

This gene encodes a member of a family of dynamin-like, integral membrane GTPases. The encoded protein is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. Mutations in this gene may be associated with hereditary sensory neuropathy type IF. Alternatively spliced transcript variants that encode distinct isoforms have been described. [provided by RefSeq, Feb 2014]

Uniprot Description

ATL3: GTPase tethering membranes through formation of trans- homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. Belongs to the GBP family. Atlastin subfamily.

Protein type: Membrane protein, multi-pass; EC 3.6.5.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: GTPase activity; identical protein binding; GTP binding

Biological Process: endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; metabolic process; Golgi organization and biogenesis; protein homooligomerization

Disease: Neuropathy, Hereditary Sensory, Type If