Rabbit anti-Human ARF1 Polyclonal Antibody | anti-ARF1 antibody

ARF1, ID (ARF1, ADP-ribosylation factor 1) (APC)

Cat. Num. AAA6274721

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARF1; Polyclonal Antibody; ID (ARF1; ADP-ribosylation factor 1) (APC); ADP-ribosylation factor 1; anti-ARF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ARF1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ARF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-114 amino acids from the Central region of human ARF1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ARF1 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARF1 antibody detected the ARF1 protein (arrow).)

Immunohistochemistry (IHC)

(ARF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ARF1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 66879662
• NCBI GeneID: 375
• NCBI Accession #: NP_001019398.1; NP_001649.1; NP_001019399.1; NP_001019397.1
• NCBI GenBank Nucleotide #: NM_001024227.1
• UniProt Accession #: P84077; P10947; P32889
• Molecular Weight: 20,697 Da
• NCBI Official Full Name: ADP-ribosylation factor 1
• NCBI Official Synonym Full Names: ADP ribosylation factor 1
• NCBI Official Symbol: ARF1
• NCBI Protein Information: ADP-ribosylation factor 1
• UniProt Protein Name: ADP-ribosylation factor 1
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF1
• UniProt Entry Name: ARF1_HUMAN

NCBI Description

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; receptor signaling protein activity

Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus