Western Blot (WB)
(APOOL Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APOOL antibody detected the APOOL protein (arrow).)
Rabbit anti-Human APOOL Polyclonal Antibody | anti-APOOL antibody
APOOL, CT (APOOL, CXorf33, FAM121A, Apolipoprotein O-like, Protein FAM121A) (Biotin)
Gene Names
APOOL; CXorf33; FAM121A; UNQ8193
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
APOOL; Polyclonal Antibody; CT (APOOL; CXorf33; FAM121A; Apolipoprotein O-like; Protein FAM121A) (Biotin); Protein FAM121A; anti-APOOL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-APOOL antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
APOOL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the C-terminal region of human APOOL.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(APOOL Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APOOL antibody detected the APOOL protein (arrow).)
Western Blot (WB)
(APOOL Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APOOL antibody detected the APOOL protein (arrow).)
Immunohistochemistry (IHC)
(APOOL Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APOOL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(APOOL Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APOOL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-APOOL antibody
References
Olsen, J.V., et al. Cell 127(3):635-648(2006). Ross, M.T., et al. Nature 434(7031):325-337(2005). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,159 Da
NCBI Official Full Name
MICOS complex subunit MIC27
NCBI Official Synonym Full Names
apolipoprotein O-like
NCBI Official Symbol
APOOL
NCBI Official Synonym Symbols
CXorf33; FAM121A; UNQ8193
NCBI Protein Information
MICOS complex subunit MIC27; AAIR8193; family with sequence similarity 121A
UniProt Protein Name
MICOS complex subunit MIC27
UniProt Gene Name
APOOL
UniProt Synonym Gene Names
CXorf33; FAM121A; MIC27
UniProt Entry Name
MIC27_HUMAN
NCBI Description
This gene encodes a protein which contains an apolipoprotein O superfamily domain. This domain is found on proteins in circulating lipoprotein complexes. [provided by RefSeq, Sep 2011]
Uniprot Description
APOOL: a protein which contains an apolipoprotein O superfamily domain. This domain is found on proteins in circulating lipoprotein complexes. [provided by RefSeq, Sep 2011]
Protein type: Secreted; Mitochondrial; Secreted, signal peptide
Chromosomal Location of Human Ortholog: Xq21.1
Cellular Component: mitochondrial inner membrane; integral to membrane
Research Articles on APOOL
Similar Products
Product Notes
The APOOL apool (Catalog #AAA6274546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APOOL, CT (APOOL, CXorf33, FAM121A, Apolipoprotein O-like, Protein FAM121A) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOOL can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the APOOL apool for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOOL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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