Western Blot (WB)
(AIDA Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the AIDA antibody detected the AIDA protein (arrow).)
Rabbit anti-Human AIDA Polyclonal Antibody | anti-AIDA antibody
AIDA, CT (AIDA, C1orf80, Axin interactor, dorsalization-associated protein, Axin interaction partner and dorsalization antagonist) (APC)
Gene Names
AIDA; C1orf80
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AIDA; Polyclonal Antibody; CT (AIDA; C1orf80; Axin interactor; dorsalization-associated protein; Axin interaction partner and dorsalization antagonist) (APC); Axin interaction partner and dorsalization antagonist; anti-AIDA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-AIDA antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
AIDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-283 amino acids from the C-terminal region of human AIDA.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(AIDA Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the AIDA antibody detected the AIDA protein (arrow).)
Western Blot (WB)
(AIDA Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the AIDA antibody detected the AIDA protein (arrow).)
Related Product Information for anti-AIDA antibody
AIDA acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity).
Product Categories/Family for anti-AIDA antibody
References
Rui, Y., et al. Dev. Cell 13(2):268-282(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,914 Da
NCBI Official Full Name
axin interactor, dorsalization-associated protein
NCBI Official Synonym Full Names
axin interactor, dorsalization associated
NCBI Official Symbol
AIDA
NCBI Official Synonym Symbols
C1orf80
NCBI Protein Information
axin interactor, dorsalization-associated protein; axin interaction partner and dorsalization antagonist
UniProt Protein Name
Axin interactor, dorsalization-associated protein
Protein Family
UniProt Gene Name
AIDA
UniProt Synonym Gene Names
C1orf80
UniProt Entry Name
AIDA_HUMAN
Uniprot Description
AIDA: Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling. Belongs to the AIDA family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: cytoplasm
Molecular Function: protein domain specific binding
Biological Process: negative regulation of JNK cascade; negative regulation of JNK activity; dorsal/ventral pattern formation; regulation of protein homodimerization activity
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Product Notes
The AIDA aida (Catalog #AAA6272378) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AIDA, CT (AIDA, C1orf80, Axin interactor, dorsalization-associated protein, Axin interaction partner and dorsalization antagonist) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AIDA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AIDA aida for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AIDA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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