Rabbit anti-Human AES Polyclonal Antibody | anti-AES antibody

AES, CT (AES, GRG, Amino-terminal enhancer of split, Gp130-associated protein GAM, Protein ESP1, Protein GRG) (PE)

Cat. Num. AAA6272013

• Gene Names: AES; GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Grg-5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AES; Polyclonal Antibody; CT (AES; GRG; Amino-terminal enhancer of split; Gp130-associated protein GAM; Protein ESP1; Protein GRG) (PE); Protein GRG; anti-AES antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-AES antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AES antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-184 amino acids from the C-terminal region of human AES.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AES (arrow) using rabbit polyclonal AES Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AES gene.)

Western Blot (WB)

(AES Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AES antibody detected the AES protein (arrow).)

Related Product Information for anti-AES antibody

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-AES antibody

Antibodies; Proteins

References

Zhang, Y., et al. Biochem. J. 427(3):499-511(2010). Beagle, B., et al. PLoS ONE 5 (7), E11821 (2010): Arce, L., et al. BMC Cancer 9, 159 (2009): Zhang, X., et al. J. Neurosci. Res. 86(11):2423-2431(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5706731
• NCBI GeneID: 166
• NCBI Accession #: NP_001121.2
• NCBI GenBank Nucleotide #: NM_001130.5
• UniProt Accession #: Q08117; Q12808; Q14CJ1; Q96TG9; Q9UDY9; B2RBL0
• Molecular Weight: 29,091 Da
• NCBI Official Full Name: amino-terminal enhancer of split isoform b
• NCBI Official Synonym Full Names: amino-terminal enhancer of split
• NCBI Official Symbol: AES
• NCBI Official Synonym Symbols: GRG; ESP1; GRG5; TLE5; AES-1; AES-2; Grg-5
• NCBI Protein Information: amino-terminal enhancer of split; gp130-associated protein GAM; groucho-related protein 5
• UniProt Protein Name: Amino-terminal enhancer of split
• Protein Family: Acetyl esterase
• UniProt Gene Name: AES
• UniProt Synonym Gene Names: GRG; GRG5; Amino enhancer of split
• UniProt Entry Name: AES_HUMAN

NCBI Description

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of growth; multicellular organismal development; cellular response to extracellular stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; skeletal development