Rabbit anti-Human ADRA2C Polyclonal Antibody | anti-ADRA2C antibody

ADRA2C, CT (ADRA2C, ADRA2L2, ADRA2RL2, Alpha-2C adrenergic receptor, Alpha-2 adrenergic receptor subtype C4, Alpha-2C adrenoreceptor) (AP)

Cat. Num. AAA6271915

• Gene Names: ADRA2C; ADRA2L2; ADRARL2; ADRA2RL2; ALPHA2CAR
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADRA2C; Polyclonal Antibody; CT (ADRA2C; ADRA2L2; ADRA2RL2; Alpha-2C adrenergic receptor; Alpha-2 adrenergic receptor subtype C4; Alpha-2C adrenoreceptor) (AP); Alpha-2C adrenoreceptor; anti-ADRA2C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ADRA2C antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ADRA2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-382 amino acids from the C-terminal region of human ADRA2C.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ADRA2C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ADRA2C antibody detected the ADRA2C protein (arrow).)

Immunohistochemistry (IHC)

(ADRA2C Antibody (C-term) (AP14582b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ADRA2C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ADRA2C antibody

Antibodies; Receptors

References

Chen, Q.J., et al. Clin. Biochem. 43(15):1201-1204(2010). Maqbool, A., et al. J. Hypertens. 28(10):2084-2093(2010). Saus, E., et al. J Psychiatr Res 44(14):971-978(2010). Gao, Y., et al. Ophthalmology (2010). In press: Montgomery, M.D., et al. Br. J. Pharmacol. 159(4):820-830(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 71773208
• NCBI GeneID: 152
• NCBI Accession #: NP_000674.2
• NCBI GenBank Nucleotide #: NM_000683.3
• UniProt Accession #: P18825; P35369; Q9HB49
• Molecular Weight: 49,522 Da
• NCBI Official Full Name: alpha-2C adrenergic receptor
• NCBI Official Synonym Full Names: adrenoceptor alpha 2C
• NCBI Official Symbol: ADRA2C
• NCBI Official Synonym Symbols: ADRA2L2; ADRARL2; ADRA2RL2; ALPHA2CAR
• NCBI Protein Information: alpha-2C adrenergic receptor; alpha-2CAR; alpha2-AR-C4; alpha-2C adrenoceptor; alpha-2C adrenoreceptor; alpha-2C-adrenergic receptor; adrenergic, alpha-2C-, receptor; alpha-2 adrenergic receptor subtype C4
• UniProt Protein Name: Alpha-2C adrenergic receptor
• Protein Family: Alpha-2C adrenergic receptor
• UniProt Gene Name: ADRA2C
• UniProt Synonym Gene Names: ADRA2L2; ADRA2RL2; Alpha-2C adrenoceptor; Alpha-2CAR
• UniProt Entry Name: ADA2C_HUMAN

NCBI Description

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]

Uniprot Description

ADRA2C: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2C sub-subfamily.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; nerve terminal; endosome

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; alpha2-adrenergic receptor activity; epinephrine binding; alpha-2A adrenergic receptor binding

Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; activation of protein kinase B; energy reserve metabolic process; positive regulation of vasoconstriction; negative regulation of epinephrine secretion; female pregnancy; regulation of sensory perception of pain; positive regulation of neuron differentiation; blood coagulation; regulation of insulin secretion