Rabbit anti-Human ADAM17 Polyclonal Antibody | anti-ADAM17 antibody

ADAM17, NT (ADAM17, CSVP, TACE, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD156b) (PE)

Cat. Num. AAA6271507

• Gene Names: ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADAM17; Polyclonal Antibody; NT (ADAM17; CSVP; TACE; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD156b) (PE); CD156b; anti-ADAM17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADAM17.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ADAM17 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 195-224 in the N-terminal region of human ADAM17, UniProt Accession #P78536.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS644197. ADAM17 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of CEM cells using MBS644197 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ADAM17 antibody

ADAM17 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM17 functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway.

Product Categories/Family for anti-ADAM17 antibody

Antibodies; CD Markers

References

1. Tellier, E., J. Cell. Physiol. 214 (3), 687-693 (2008). 2. Takamune, Y., Biochem. Biophys. Res. Commun. 365 (2), 393-398 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 73747889
• NCBI GeneID: 6868
• NCBI Accession #: NP_003174.3
• NCBI GenBank Nucleotide #: NM_003183.4
• UniProt Accession #: P78536; O60226
• Molecular Weight: 93,021 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 17 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 17
• NCBI Official Symbol: ADAM17
• NCBI Official Synonym Symbols: CSVP; TACE; NISBD; ADAM18; CD156B
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM17
• UniProt Synonym Gene Names: CSVP; TACE; ADAM 17
• UniProt Entry Name: ADA17_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.4.24.86; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft

Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; cell motility involved in cell locomotion; neutrophil mediated immunity; T cell differentiation in the thymus; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; response to drug; spleen development; epidermal growth factor receptor signaling pathway; Notch signaling pathway; membrane protein intracellular domain proteolysis; response to high density lipoprotein stimulus; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; collagen catabolic process; regulation of mast cell apoptosis; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; wound healing, spreading of epidermal cells; positive regulation of cell migration

Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1