Rabbit anti-Human Connexin 31.1 Polyclonal Antibody | anti-GJB5 antibody

Connexin 31.1, ID (Gap Junction beta-5 Protein, Connexin-31.1, Cx31.1)

Cat. Num. AAA627099

• Gene Names: GJB5; CX31.1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Connexin 31.1; Polyclonal Antibody; ID (Gap Junction beta-5 Protein; Connexin-31.1; Cx31.1); Anti -Connexin 31.1; anti-GJB5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJB5.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-GJB5 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the center region of human GJB5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(GJB5 Antibody (R104.Connexin) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GJB5 antibody detected the GJB5 protein (arrow).)

Related Product Information for anti-GJB5 antibody

Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) such as GJB5 are thought to play crucial functional roles associated with these channels.

Product Categories/Family for anti-GJB5 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4336903
• NCBI GeneID: 2709
• UniProt Accession #: O95377; Q9UPA3
• Molecular Weight: 31,088 Da
• NCBI Official Full Name: connexin 31.1
• NCBI Official Synonym Full Names: gap junction protein, beta 5, 31.1kDa
• NCBI Official Symbol: GJB5
• NCBI Official Synonym Symbols: CX31.1
• NCBI Protein Information: gap junction beta-5 protein; connexin 31.1; connexin-31.1; OTTHUMP00000004186; gap junction protein, beta 5 (connexin 31.1)
• UniProt Protein Name: Gap junction beta-5 protein
• Protein Family: Gap junction beta-5 protein
• UniProt Gene Name: GJB5
• UniProt Entry Name: CXB5_HUMAN

NCBI Description

Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family.[supplied by OMIM]

Uniprot Description

GJB5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: connexon complex; integral to membrane

Biological Process: epidermis development; cell communication