Mouse anti-Bovine CD4 Monoclonal Antibody | anti-CD4 antibody

CD4 (APC)

Cat. Num. AAA6268467

• Reactivity: Bovine
• Applications: Immunohistochemistry, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: CD4; Monoclonal Antibody; CD4 (APC); anti-CD4 antibody

• Host: Mouse
• Reactivity: Bovine
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: CC8
• Specificity: Recognizes bovine CD4.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CD4 antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Bovine lymphocytes.
• Conjugate: APC
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:Alexa Fluor® 647)

Testing Data

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:FITC)

Product Categories/Family for anti-CD4 antibody

Antibodies; CD Markers

References

1. Bensaid, A. et al. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4). Vet. Immunol. Immunopathol. 27: 51-54. 2. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-231. 3. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle. Vet. Immunol. Immunopathol. 27: 21-27. 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 5. Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex. Infect Immun. 76: 3932-9. 6. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus. J Virol. 79: 7738-44. 7. Buddle, B.M. et al. (2003) Revaccination of neonatal calves with Mycobacterium bovis BCG reduces the level of protection against bovine tuberculosis induced by a single vaccination. Infect Immun. 71: 6411-9. 8. Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes. J Virol. 83: 4039-50. 9. Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells Immunology. 105: 190-5. 10. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves. BMC Vet Res. 6: 39. 11. Hu, X.D. et al. (2009) Immunotherapy with combined DNA vaccines is an effective treatment for M. bovis infection in cattle Vaccine. 27: 1317-22. 12. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses. Vet Res. 41: 14. 13. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS One. 6: e29194. 14. Wernike, K. et al. (2013) Oral exposure, reinfection and cellular immunity to Schmallenberg virus in cattle. Vet Microbiol. pii: S0378-1135(13)00092-8. 15. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving. Vet Res Commun. 34: 197-203. 16. Dacal,. V. et al. (2013) Immunohistochemical characterization of inflammatory cells in the skin of cattle undergoing repeated infestations with Hypoderma lineatum (Diptera: Oestridae) larvae. J Comp Pathol. 145: 282-8. 17. Oh, Y. et al. (2012) Interferon-gamma induced by in vitro re-stimulation of CD4+ T-cells correlates with in vivo FMD vaccine induced protection of cattle against disease and persistent infection. PLoS One. 7: e44365. 18. Hine, B.C. et al. (2012) Analysis of leukocyte populations in Canadian Holsteins classified as high or low immune responders for antibody- or cell-mediated immune response. Can J Vet Res. 76: 149-56. 19. Aranday-Cortes, E. et al. (2012) Transcriptional profiling of disease-induced host responses in bovine tuberculosis and the identification of potential diagnostic biomarkers. PLoS One. 7: e30626. 20. Tenaya, I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus. Vet Immunol Immunopathol. 149: 167-76. 21. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis. Theriogenology 28 April

NCBI and Uniprot Product Information

• NCBI GI #: 157073972
• NCBI GeneID: 407098
• NCBI Accession #: NP_001096695.1
• NCBI GenBank Nucleotide #: NM_001103225.1
• UniProt Accession #: A7YY52
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Symbol: CD4
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 antigen
• UniProt Protein Name: CD4 protein
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: A7YY52_BOVIN