Rabbit anti-Human USP22 Polyclonal Antibody | anti-USP22 antibody

USP22, NT (Ubiquitin Carboxyl-terminal Hydrolase 22, Ubiquitin Thiolesterase 22, Ubiquitin-specific Processing Protease 22, Deubiquitinating Enzyme 22, KIAA1063, USP3L)

Cat. Num. AAA626272

• Gene Names: USP22; USP3L; KIAA1063
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: USP22; Polyclonal Antibody; NT (Ubiquitin Carboxyl-terminal Hydrolase 22; Ubiquitin Thiolesterase 22; Ubiquitin-specific Processing Protease 22; Deubiquitinating Enzyme 22; KIAA1063; USP3L); Anti -USP22; anti-USP22 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP22.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-USP22 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human USP22 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of USP22 Antibody (N-term) in Jurkat cell line lysates (35ug/lane).USP22(arrow) was detected using the purified Pab.)

Related Product Information for anti-USP22 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP22 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 150010639
• NCBI GeneID: 23326
• NCBI Accession #: NP_056091.1
• NCBI GenBank Nucleotide #: NM_015276.1
• UniProt Accession #: Q9UPT9; Q2NLE2; Q6MZY4; Q8TBS8; Q96IW5; A0JNS3; Q9NSJ7
• Molecular Weight: 59,961 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 22
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 22
• NCBI Official Symbol: USP22
• NCBI Official Synonym Symbols: USP3L; KIAA1063
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 22; OTTHUMP00000164990; ubiquitin thioesterase 22; deubiquitinating enzyme 22; ubiquitin thiolesterase 22; ubiquitin specific protease 22; ubiquitin-specific processing protease 22; ubiquitin-specific-processing protease 22
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 22
• UniProt Gene Name: USP22
• UniProt Synonym Gene Names: KIAA1063; USP3L
• UniProt Entry Name: UBP22_HUMAN

Uniprot Description

USP22: Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression. Belongs to the peptidase C19 family. UBP8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Cell cycle regulation; Transcription, coactivator/corepressor; Ubiquitin-specific protease; Ubiquitin conjugating system; Nuclear receptor co-regulator; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 17p11.2

Molecular Function: H4 histone acetyltransferase activity; protein binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; zinc ion binding; cysteine-type endopeptidase activity; transcription coactivator activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; embryonic development; protein deubiquitination; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone ubiquitination; positive regulation of mitotic cell cycle; cell cycle; histone deubiquitination