Mouse Claudin 1 Monoclonal Antibody | anti-CLDN1 antibody

Claudin 1 (CLDN1) (HRP)

Cat. Num. AAA6258794

• Gene Names: CLDN1; CLD1; SEMP1; ILVASC
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: Claudin 1; Monoclonal Antibody; Claudin 1 (CLDN1) (HRP); anti-CLDN1 antibody

• Host: Mouse
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6A42
• Specificity: Recognizes the C-terminal region of the human claudin-1 protein. On Western Blots, it identifies the target band at ~22kD. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates, mouse kidney and intestinal lysates, frozen mouse intestine (jejunum) tissue, and rat kidney homogenates.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-CLDN1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to the C-terminal region of the human and mouse claudin-1 proteins.
• Conjugate: HRP
• Preparation and Storage: Store at -20 degree C.

Immunofluorescence (IF)

(Indirect immunofluorescence staining of frozen mouse jejunum tissue using C5838-02A. ZO-1 is labeled in green; nuclei are stained with DAPI (blue).)

Immunofluorescence (IF)

(Indirect immunofluorescence staining of MDCK cells using C5838-02A.)

Product Categories/Family for anti-CLDN1 antibody

Antibodies; Claudin

References

1. Heiskala, M., et al., Traffic 2(2): 93-98 (2001). 2. Furuse, M., et al., J. Cell. Biol. 143(2): 391-401 (1998). 3. Kramer, F., et al., Hum. Genet. 107: 249-256 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 10863887
• NCBI GeneID: 9076
• NCBI Accession #: NP_066924.1
• NCBI GenBank Nucleotide #: NM_021101.4
• UniProt Accession #: O95832
• Molecular Weight: 22,744 Da
• NCBI Official Full Name: claudin-1
• NCBI Official Synonym Full Names: claudin 1
• NCBI Official Symbol: CLDN1
• NCBI Official Synonym Symbols: CLD1; SEMP1; ILVASC
• NCBI Protein Information: claudin-1; senescence-associated epithelial membrane protein 1
• UniProt Protein Name: Claudin-1
• Protein Family: Claudin
• UniProt Gene Name: CLDN1
• UniProt Synonym Gene Names: CLD1; SEMP1
• UniProt Entry Name: CLD1_HUMAN

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

Claudin-1: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Acts as a co- receptor for HCV entry into hepatic cells. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL. May interact with HCV E1 and E2 proteins. Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. Belongs to the claudin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cytoskeletal

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: tight junction; integral to plasma membrane; apical plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: identical protein binding; protein binding; structural molecule activity

Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell adhesion; calcium-independent cell-cell adhesion

Disease: Ichthyosis, Leukocyte Vacuoles, Alopecia, And Sclerosing Cholangitis