Dot Blot (DB)
(Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit anti-Human ERK1 Polyclonal Antibody
ERK1, phosphorylated (Thr202/Tyr204) (Extracellular Signal-regulated Kinase 1, ERK-1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Microtubule-associated Protein 2 Kinase, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, MAPK3, M
Reactivity
Human
Applications
ELISA, Western Blot, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ERK1; Polyclonal Antibody; phosphorylated (Thr202/Tyr204) (Extracellular Signal-regulated Kinase 1; ERK-1; ERT2; Insulin-stimulated MAP2 Kinase; MAP Kinase Isoform p44; Microtubule-associated Protein 2 Kinase; Mitogen-activated Protein Kinase 3; MAP Kinase 3; MAPK 3; MAPK3; M; Anti -ERK1; anti-ERK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ERK1 when phosphorylated at Thr202 and Tyr204.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-ERK1 antibody
ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA, Western Blot, and Dot Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Dot Blot: 1:500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Dot Blot: 1:500
Immunogen
Synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated Thr202 and Tyr204 of human ERK1 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Dot Blot (DB)
(Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Dot Blot (DB)
(Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Western Blot (WB)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) . HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane).)
Western Blot (WB)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) . HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane).)
Western Blot (WB)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) . HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane) .)
Western Blot (WB)
(Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) . HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane) .)
Related Product Information for anti-ERK1 antibody
MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Product Categories/Family for anti-ERK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Extracellular signal-regulated kinase 1
Similar Products
Product Notes
The ERK1 (Catalog #AAA625402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERK1, phosphorylated (Thr202/Tyr204) (Extracellular Signal-regulated Kinase 1, ERK-1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Microtubule-associated Protein 2 Kinase, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, MAPK3, M reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA, Western Blot, and Dot Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Dot Blot: 1:500. Researchers should empirically determine the suitability of the ERK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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