Goat anti-Rabbit IgG, H&L, X-Adsorbed Polyclonal Antibody
IgG, H&L, X-Adsorbed (AP)
Reactivity
Rabbit
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purified~90% based on SDS-PAGE, small amounts of intact IgG may be present.
Synonyms
IgG; H&L; X-Adsorbed; Polyclonal Antibody; X-Adsorbed (AP); Anti -IgG; anti-IgG; X-Adsorbed antibody
Host
Goat
Reactivity
Rabbit
Clonality
Polyclonal
Isotype
F(ab')2
Specificity
Recognizes heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on IEP. Does not recognize non-immunoglobulin rabbit serum proteins. Does not recognize serum proteins from bovine, human, or mouse or IgG from human or mouse.
Purity/Purification
Affinity Purified
~90% based on SDS-PAGE, small amounts of intact IgG may be present.
~90% based on SDS-PAGE, small amounts of intact IgG may be present.
Form/Format
Supplied as a liquid in 30mM triethanolamine, pH7.2, 5mM magnesium chloride, 0.1mM zinc chloride, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-IgG, H&L, X-Adsorbed antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: ELISA: 1:500-1:2000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Dilution: ELISA: 1:500-1:2000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Immunogen
Purified Rabbit IgG, whole molecule
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.
Product Categories/Family for anti-IgG, H&L, X-Adsorbed antibody
Similar Products
Product Notes
The IgG, H&L, X-Adsorbed (Catalog #AAA624741) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The IgG, H&L, X-Adsorbed (AP) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's IgG, H&L, X-Adsorbed can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: ELISA: 1:500-1:2000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000. Researchers should empirically determine the suitability of the IgG, H&L, X-Adsorbed for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, H&L, X-Adsorbed, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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