Rabbit anti-Human Keratinocyte Growth Factor Polyclonal Antibody | anti-FGF7 antibody

Keratinocyte Growth Factor (KGF, Fibroblast Growth Factor 7, FGF-7)

Cat. Num. AAA624653

• Gene Names: FGF7; KGF; HBGF-7
• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Keratinocyte Growth Factor; Polyclonal Antibody; Keratinocyte Growth Factor (KGF; Fibroblast Growth Factor 7; FGF-7); Anti -Keratinocyte Growth Factor (KGF; anti-FGF7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KGF.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile 40-50% glycerol, ddH2O to a concentration of 0.1-1.0mg/ml.

• Applicable Applications for anti-FGF7 antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in ELISA, Neutralization, and Western Blot.; Dilution: ELISA (Indirect): 0.5-2ug/ml. Using 100ul/well antibody solution. When used in conjunction with the compatible secondary reagents, the detection limit is of 0.2-0.4ng/well.; ELISA (Sandwich): 0.5-2ug/ml. Using 100ul/well antibody solution. When used in conjunction with K1000A, as a detection antibody, the detection limit is of 0.2-0.4ng/well.; Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.; Immunohistochemistry: 0.5ug/ml, overnight at 4 degree C. This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. An HRP-labeled polymerdetection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.; Neutralization: 0.56-0.85ug/ml, to yeild one-half maximal inhibition [ND50] of the biological activity of hKGF (25ng/ml).; Optimal dilution determined by the researcher.
• Immunogen: Highly purified (>98%) recombinant antigen.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-FGF7 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4503705
• NCBI GeneID: 2252
• NCBI Accession #: NP_002000.1
• NCBI GenBank Nucleotide #: NM_002009.3
• UniProt Accession #: P21781; Q6FGV5; Q6RK68; Q96FG5
• Molecular Weight: 22,509 Da
• NCBI Official Full Name: keratinocyte growth factor
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: FGF7
• NCBI Official Synonym Symbols: KGF; HBGF-7
• NCBI Protein Information: keratinocyte growth factor; FGF-7; OTTHUMP00000162252; heparin-binding growth factor 7
• UniProt Protein Name: Keratinocyte growth factor
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF7
• UniProt Synonym Gene Names: KGF
• UniProt Entry Name: FGF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq]

Uniprot Description

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; epidermis development; hair follicle morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; actin cytoskeleton reorganization; response to wounding; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation