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Western Blot (WB) (The anti-PACSIN2 Pab is used in Western blot to detect PACSIN2 in T-47D cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2).)

Rabbit anti-Human, Mouse PACSIN2 Polyclonal Antibody | anti-PACSIN2 antibody

PACSIN2, CT (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2)

Gene Names
PACSIN2; SDPII
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
PACSIN2; Polyclonal Antibody; CT (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2); Anti -PACSIN2; anti-PACSIN2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PACSIN2. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-PACSIN2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:50-1:100
Immunogen
Synthetic peptide selected from the C-terminal region of human PACSIN2 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PACSIN2 Pab is used in Western blot to detect PACSIN2 in T-47D cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2).)

Western Blot (WB) (The anti-PACSIN2 Pab is used in Western blot to detect PACSIN2 in T-47D cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-PACSIN2 antibody
PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.
Product Categories/Family for anti-PACSIN2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,739 Da
NCBI Official Full Name
protein kinase C and casein kinase substrate in neurons protein 2 isoform A
NCBI Official Synonym Full Names
protein kinase C and casein kinase substrate in neurons 2
NCBI Official Symbol
PACSIN2
NCBI Official Synonym Symbols
SDPII
NCBI Protein Information
protein kinase C and casein kinase substrate in neurons protein 2; syndapin II; OTTHUMP00000198281; OTTHUMP00000198282; OTTHUMP00000198283; OTTHUMP00000198284; cytoplasmic phosphoprotein PACSIN2
UniProt Protein Name
Protein kinase C and casein kinase substrate in neurons protein 2
UniProt Gene Name
PACSIN2
UniProt Entry Name
PACN2_HUMAN

NCBI Description

This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

PACSIN2: May play a role in vesicle formation and transport. Belongs to the PACSIN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.2-q13.33

Cellular Component: nucleoplasm; cytoplasmic vesicle membrane; extrinsic to membrane; cytoskeleton; focal adhesion; intracellular membrane-bound organelle; recycling endosome membrane; cytoplasm; early endosome; caveola; intercellular junction; cytosol

Molecular Function: identical protein binding; protein binding; transporter activity; cytoskeletal protein binding

Biological Process: negative regulation of endocytosis; regulation of endocytosis; cell projection morphogenesis; actin cytoskeleton organization and biogenesis

Research Articles on PACSIN2

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Product Notes

The PACSIN2 pacsin2 (Catalog #AAA624569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PACSIN2, CT (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PACSIN2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:50-1:100. Researchers should empirically determine the suitability of the PACSIN2 pacsin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PACSIN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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