Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using PACSIN2 Antibody (C-term). MBS9211841 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Rabbit anti-Human, mouse PACSIN2 Polyclonal Antibody | anti-PACSIN2 antibody
PACSIN2 Antibody (C-term)
IF~~1:100
WB~~1:1000
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using PACSIN2 Antibody (C-term). MBS9211841 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using PACSIN2 Antibody (C-term). MBS9211841 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.stomach section using PACSIN2 Antibody (C-term). MBS9211841 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H.stomach section using PACSIN2 Antibody (C-term). MBS9211841 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term). MBS9211841 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term). MBS9211841 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Western Blot (WB)
(PACSIN2 Antibody (S357) western blot analysis in Daudi cell line and mouse brain lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).)
Western Blot (WB)
(PACSIN2 Antibody (S357) western blot analysis in Daudi cell line and mouse brain lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).)
Western Blot (WB)
(PACSIN2 Antibody (S357) western blot analysis in Daudi,HL-60 cell line and mouse brain,rat heart lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).)
Western Blot (WB)
(PACSIN2 Antibody (S357) western blot analysis in Daudi,HL-60 cell line and mouse brain,rat heart lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Uniprot Description
PACSIN2: May play a role in vesicle formation and transport. Belongs to the PACSIN family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 22q13.2-q13.33
Cellular Component: nucleoplasm; cytoplasmic vesicle membrane; cytoskeleton; extrinsic to membrane; focal adhesion; intracellular membrane-bound organelle; recycling endosome membrane; early endosome; cytoplasm; caveola; intercellular junction; cytosol
Molecular Function: identical protein binding; protein binding; transporter activity; cytoskeletal protein binding
Biological Process: negative regulation of endocytosis; regulation of endocytosis; cell projection morphogenesis; actin cytoskeleton organization and biogenesis
Research Articles on PACSIN2
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Product Notes
The PACSIN2 pacsin2 (Catalog #AAA9211841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 342-371. The PACSIN2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PACSIN2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF). IHC~~1:50~100 IF~~1:100 WB~~1:1000. Researchers should empirically determine the suitability of the PACSIN2 pacsin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PACSIN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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