Rabbit anti-Human MOBKL1B Polyclonal Antibody | anti-MOBKL1B antibody

MOBKL1B, CT (Mps One Binder Kinase Activator-like 1B, Mob1 Homolog 1B, MOBK1B, C2orf6, FLJ10788, FLJ11595, MATS1, MOB4B, Protein Mob4B)

Cat. Num. AAA624334

• Gene Names: MOBKL1B; MOB1; MATS1; Mob4B; C2orf6; MOBK1B; FLJ10788; FLJ11595
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: MOBKL1B; Polyclonal Antibody; CT (Mps One Binder Kinase Activator-like 1B; Mob1 Homolog 1B; MOBK1B; C2orf6; FLJ10788; FLJ11595; MATS1; MOB4B; Protein Mob4B); Anti -MOBKL1B; anti-MOBKL1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MOBKL1B.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MOBKL1B antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human MOBKL1B (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MOBKL1B antibody (C-term) in A2058 cell line lysates (35ug/lane). MOBKL1B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(MOBKL1B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MOBKL1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of MOBKL1B (arrow) using rabbit polyclonal MOBKL1B Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MOBKL1B gene.)

Flow Cytometry (FC/FACS)

(MOBKL1B Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MOBKL1B antibody

MOBKL1B stimulates the kinase activity of STK38 and STK38L.

Product Categories/Family for anti-MOBKL1B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 148747437
• NCBI GeneID: 55233
• NCBI Accession #: NP_060691.2
• NCBI GenBank Nucleotide #: NM_018221.3
• UniProt Accession #: Q9H8S9; Q53S34; Q9H3T5; Q9HAI0; Q9NVE2
• Molecular Weight: 25,080 Da
• NCBI Official Full Name: mps one binder kinase activator-like 1B
• NCBI Official Synonym Full Names: MOB1, Mps One Binder kinase activator-like 1B (yeast)
• NCBI Official Symbol: MOBKL1B
• NCBI Official Synonym Symbols: MOB1; MATS1; Mob4B; C2orf6; MOBK1B; FLJ10788; FLJ11595
• NCBI Protein Information: mps one binder kinase activator-like 1B; mob1A; mob1 alpha; mob1 homolog 1B; OTTHUMP00000202550
• UniProt Protein Name: Mps one binder kinase activator-like 1B
• UniProt Gene Name: MOBKL1B
• UniProt Synonym Gene Names: C2orf6; MOB4B; MOBK1B
• UniProt Entry Name: MOL1B_HUMAN

Uniprot Description

MOB1A: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38. Binds STK38 and STK38L. Interacts with LATS1 and LATS2. Forms a tripartite complex with STK38 and STK3/MST2. Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cytosol

Molecular Function: protein binding; metal ion binding