Sheep anti-Human, Mouse Nogo-B Polyclonal Antibody | anti-Rtn4 antibody

Nogo-B (Foocen, KIAA0886, My043, Nbla00271, Nbla10545, Neurite Outgrowth Inhibitor, Neuroendocrine-specific Protein, NI220/250, NSP, NSP-CL, Reticulon-4, Reticulon-5, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, RTN-x, RTN-X, SP1507, SP1507)

Cat. Num. AAA624324

• Gene Names: Rtn4; ASY; NgA; NOGO; NSP-CL; Nogo-A; Nogo-B; Nogo-C; AA407876; AA409940; AA960376; mKIAA0886; mKIAA4153; 1110020G17Rik; C130026I10Rik
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Nogo-B; Polyclonal Antibody; Nogo-B (Foocen; KIAA0886; My043; Nbla00271; Nbla10545; Neurite Outgrowth Inhibitor; Neuroendocrine-specific Protein; NI220/250; NSP; NSP-CL; Reticulon-4; Reticulon-5; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; RTN-x; RTN-X; SP1507; SP1507); Anti -Nogo-B (Foocen; anti-Rtn4 antibody

• Host: Sheep
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Nogo-B. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-Rtn4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1ug/ml; ELISA: 0.5-1ug/ml
• Immunogen: CHO cell-derived, recombinant human Nogo-B (rhNogo-B; aa1-200; Accession #NP_722550).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.02% NaN3. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of Nogo-B: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-h/mNogo-B.)

Related Product Information for anti-Rtn4 antibody

Nogo-B (A No-Go for neurite outgrowth Isoform B; also reticulon-4 isoform 2 and RTN-xS) is a 49-51kD member of the reticulon protein family. It is widely expressed, being reported everywhere but liver. Nogo-B appears to be principally expressed in the ER, but does have a receptor (NgBR) on cell surfaces. Intracellularly, Nogo-B is known to interact with Bcl-xl and Bcl-2, and may play in role in both apoptosis and angiogenesis. Human Nogo-B is a two transmembrane, 373aa protein. It contains an N-terminal cytoplasmic domain (aa1-199), two transmembrane segments (aa200-219 and 315-335), a luminal region (aa220-314) and a C-terminal cytoplasmic domain (aa336-373). Nogo-B contains multiple phosphorylation sites and undergoes acetylation. Caspase-7 cleaves Nogo-B between Asp14Ser15, generating a 45-47kD fragment. Nogo-B is generated from a full-length 205kD Nogo-A by the deletion ofaa186-1004. Overaa1-200, human Nogo-B shares 87% aa identity with mouse Nogo-B1.

Product Categories/Family for anti-Rtn4 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21898579
• NCBI GeneID: 68585
• UniProt Accession #: Q99P72; Q5DTK9; Q7TNB7; Q80W95; Q8BGK7; Q8BGM9; Q8K290; Q8K3G8; Q9CTE3
• Molecular Weight: 126,613 Da
• NCBI Official Full Name: nogo-B
• NCBI Official Synonym Full Names: reticulon 4
• NCBI Official Symbol: Rtn4
• NCBI Official Synonym Symbols: ASY; NgA; NOGO; NSP-CL; Nogo-A; Nogo-B; Nogo-C; AA407876; AA409940; AA960376; mKIAA0886; mKIAA4153; 1110020G17Rik; C130026I10Rik
• NCBI Protein Information: reticulon-4; neurite outgrowth inhibitor
• UniProt Protein Name: Reticulon-4
• Protein Family: Reticulon
• UniProt Gene Name: Rtn4
• UniProt Synonym Gene Names: Kiaa0886; Nogo; Nogo protein
• UniProt Entry Name: RTN4_MOUSE

Uniprot Description

Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Cell development/differentiation; Inhibitor; Membrane protein, integral; Motility/polarity/chemotaxis; Endoplasmic reticulum

Cellular Component: neuron projection; protein complex; endoplasmic reticulum; integral to membrane; nuclear envelope; cell projection; membrane; cell soma; cytoplasm; plasma membrane; integral to endoplasmic reticulum membrane; intracellular; myelin sheath

Molecular Function: protein binding; protein complex binding

Biological Process: positive regulation of dopamine secretion; nervous system development; negative regulation of neuron differentiation; cerebral cortex radial glia guided migration; regulation of nervous system development; axonal fasciculation; positive regulation of glial cell differentiation; negative regulation of axonogenesis; angiogenesis; regulation of sensory perception of pain; negative regulation of axon extension; negative regulation of cell growth; regulation of cell migration