Western Blot (WB)
(Western Blot Analysis: 5-10ug/ml of this lot detected 10ng PLA2 under non-reducing conditions. Western Blot Analysis Human synovial PLA2 was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS601861 (5ug/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PLA2.)
Mouse anti-Human Phospholipase A2 Monoclonal Antibody | anti-sPLA2 antibody
Phospholipase A2, Secretory (PLA2, sPLA2) (Biotin)
Western Blot (WB)
(Western Blot Analysis: 5-10ug/ml of this lot detected 10ng PLA2 under non-reducing conditions. Western Blot Analysis Human synovial PLA2 was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS601861 (5ug/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PLA2.)
Western Blot (WB)
(Western Blot Analysis: 5-10ug/ml of this lot detected 10ng PLA2 under non-reducing conditions. Western Blot Analysis Human synovial PLA2 was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS601861 (5ug/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PLA2.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]
Uniprot Description
PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.
Protein type: Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Vesicle; Endoplasmic reticulum; Phospholipase; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Mitochondrial; Secreted; Lipid Metabolism - linoleic acid
Chromosomal Location of Human Ortholog: 1p35
Cellular Component: extracellular space; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; extracellular region; secretory granule
Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; phospholipid binding; calcium ion binding
Biological Process: defense response to Gram-positive bacterium; phosphatidic acid metabolic process; somatic stem cell maintenance; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process; positive regulation of inflammatory response; negative regulation of epithelial cell proliferation
Disease: Colorectal Cancer
Research Articles on sPLA2
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Product Notes
The sPLA2 pla2g2a (Catalog #AAA6242565) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Phospholipase A2, Secretory (PLA2, sPLA2) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase A2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the sPLA2 pla2g2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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