Rabbit AFX Polyclonal Antibody | anti-FOXO4 antibody

AFX (Forkhead Box Protein O4, Fork Head Domain Transcription Factor AFX1, FOXO4, AFX1, MLLT7)

Cat. Num. AAA624202

• Gene Names: FOXO4; AFX; AFX1; MLLT7; MGC120490
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AFX; Polyclonal Antibody; AFX (Forkhead Box Protein O4; Fork Head Domain Transcription Factor AFX1; FOXO4; AFX1; MLLT7); Anti -AFX (Forkhead Box Protein O4; anti-FOXO4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AFX. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-FOXO4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot:1:500-1:1,000
• Immunogen: Synthetic non-phosphopeptide corresponding to human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-FOXO4 antibody

Several studies indicate that FKHR and AFX, mammalian homologues of the Caenorhabditis elegans forkhead transcription factor DAF-16, function in the insulin signaling pathway promoter contains one IRS, while in the IGFBP-1 promoter two copies of IRS are arranged as an inverted palindrome. Three tandem copies of IRSs are present in the G6Pase. AFX has been recently shown to serve as a substrate of PKB both in vitro and in vivo, and its transcriptional activity is inhibited by phosphorylation.

Product Categories/Family for anti-FOXO4 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1418759
• NCBI GeneID: 4303
• UniProt Accession #: P98177; O43821; Q13720; Q3KPF1; Q8TDK9; B7WPJ7
• Molecular Weight: 53,684 Da
• NCBI Official Full Name: AFX
• NCBI Official Synonym Full Names: forkhead box O4
• NCBI Official Symbol: FOXO4
• NCBI Official Synonym Symbols: AFX; AFX1; MLLT7; MGC120490
• NCBI Protein Information: forkhead box protein O4; OTTHUMP00000023496; OTTHUMP00000023497; fork head domain transcription factor AFX1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
• UniProt Protein Name: Forkhead box protein O4
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO4
• UniProt Synonym Gene Names: AFX; AFX1; MLLT7
• UniProt Entry Name: FOXO4_HUMAN

NCBI Description

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; epidermal growth factor receptor signaling pathway; muscle development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell differentiation; stem cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; insulin receptor signaling pathway; innate immune response; cell cycle arrest