Rabbit anti-Human USP19 Polyclonal Antibody | anti-USP19 antibody

USP19, CT (Ubiquitin Thiolesterase 19, Ubiquitin-specific Processing Protease 19, Deubiquitinating Enzyme 19, Ubiquitin Carboxyl-terminal Hydrolase 19, KIAA0891, ZMYND9)

Cat. Num. AAA624138

• Gene Names: USP19; ZMYND9
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: USP19; Polyclonal Antibody; CT (Ubiquitin Thiolesterase 19; Ubiquitin-specific Processing Protease 19; Deubiquitinating Enzyme 19; Ubiquitin Carboxyl-terminal Hydrolase 19; KIAA0891; ZMYND9); Anti -USP19; anti-USP19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP19.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-USP19 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human USP19 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hUSP19-L1304 in Jurkat cell line lysates (35ug/lane). USP19 (arrow) was detected using the purified Pab.)

Related Product Information for anti-USP19 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP19 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 312596871
• NCBI GeneID: 10869
• NCBI Accession #: NP_001186089.1
• NCBI GenBank Nucleotide #: NM_001199160.1
• UniProt Accession #: O94966; Q3KQW4; Q641Q9; Q6NZY8; Q86XV9; A6H8U2
• Molecular Weight: 145,651 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 19 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 19
• NCBI Official Symbol: USP19
• NCBI Official Synonym Symbols: ZMYND9
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 19; OTTHUMP00000164974; OTTHUMP00000164975; OTTHUMP00000164977; OTTHUMP00000164978; OTTHUMP00000210659; OTTHUMP00000210660; OTTHUMP00000210661; ubiquitin thioesterase 19; deubiquitinating enzyme 19; ubiquitin thiolesterase 19; ubiquitin specific protease 19; ubiquitin-specific-processing protease 19; zinc finger MYND domain-containing protein 9
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 19
• UniProt Gene Name: USP19
• UniProt Synonym Gene Names: KIAA0891; ZMYND9
• UniProt Entry Name: UBP19_HUMAN

Uniprot Description

USP19: Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin- dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic- reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non- catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains. Belongs to the peptidase C19 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Membrane protein, integral; EC 3.4.19.12; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: endoplasmic reticulum membrane; integral to membrane; cytosol

Molecular Function: protein binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: negative regulation of skeletal muscle development; ER-associated protein catabolic process; protein deubiquitination; regulation of protein stability