Rabbit Gelatin, Skin Polyclonal Antibody
Anti-Gelatin, Skin
Applications
ELISA
Purity
Serum
Synonyms
Gelatin; Skin; Polyclonal Antibody; Anti-Gelatin; anti-Gelatin; Skin antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes fish gelatin.
Purity/Purification
Serum
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Applicable Applications for anti-Gelatin, Skin antibody
ELISA
Application Notes
Recommended Dilutions: ELISA: 1:1000-1:10,000
Immunogen
Gelatin, from cold water fish.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-Gelatin, Skin antibody
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue and Type B gelatin is derived from lime-cured tissue. The charge on a gelatin molecule and its isoelectric point are primarily due to the carboxyl amino and guanidino groups on the side chains. Type A gelatin has 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.0-9.0; type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2. The pH of a 1.5% solution at 25 degree C is 3.8-5.5 for Type A and 5.0-7.5 for Type B. soluble in glycerol and acetic acid, and more soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils.
Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to help stabilize Taq DNA polymerase, and use as a blocking reagent in Western blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media for species differentiation. Additionally, as a biocompatible polymer, gelatin has been used as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for tissue engineering applications. Industrial applications include the use of gelatin as a stabilizer, thickener, and texturizer in foods and in the manufacture of rubber substitutes, adhesives, cements, lithographic and printing inks, plastic compounds, artificial silk, photographic plates and films, matches, and light filters for mercury lamps. In the pharmaceutical industry, gelatin is used as a suspending agent, encapsulating agent, and tablet binder; and in veterinary applications it is used as a plasma expander and hemostatic sponge.
Gelatin proteins are also used as additives in purified, recombinant proteins and antibodies. Animals or humans exposed to gelatins may invoke antibody response. Often the gelatin must be removed prior to such applications. Antibodies to gelatins were produced to detect the presence of gelatins in biolgocial samples and or to verify their removal during bioprocess development.
Many people wish to avoid taking animal derived gelatin due to alltergy and for religious or ethical reasons. The antibodies to gelatins can be used to detec the presence of gelatin.
Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to help stabilize Taq DNA polymerase, and use as a blocking reagent in Western blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media for species differentiation. Additionally, as a biocompatible polymer, gelatin has been used as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for tissue engineering applications. Industrial applications include the use of gelatin as a stabilizer, thickener, and texturizer in foods and in the manufacture of rubber substitutes, adhesives, cements, lithographic and printing inks, plastic compounds, artificial silk, photographic plates and films, matches, and light filters for mercury lamps. In the pharmaceutical industry, gelatin is used as a suspending agent, encapsulating agent, and tablet binder; and in veterinary applications it is used as a plasma expander and hemostatic sponge.
Gelatin proteins are also used as additives in purified, recombinant proteins and antibodies. Animals or humans exposed to gelatins may invoke antibody response. Often the gelatin must be removed prior to such applications. Antibodies to gelatins were produced to detect the presence of gelatins in biolgocial samples and or to verify their removal during bioprocess development.
Many people wish to avoid taking animal derived gelatin due to alltergy and for religious or ethical reasons. The antibodies to gelatins can be used to detec the presence of gelatin.
Product Categories/Family for anti-Gelatin, Skin antibody
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Product Notes
The Gelatin, Skin (Catalog #AAA623692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Gelatin, Skin can be used in a range of immunoassay formats including, but not limited to, ELISA. Recommended Dilutions: ELISA: 1:1000-1:10,000. Researchers should empirically determine the suitability of the Gelatin, Skin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gelatin, Skin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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