Rabbit anti-Human PDX1 Polyclonal Antibody | anti-PDX1 antibody

PDX1, phosphorylated (Thr11) (Pancreas/Duodenum Homeobox Protein 1, Insulin Promoter Factor 1, Islet/Duodenum Homeobox-1, Somatostatin-transactivating Factor 1, Insulin Upstream Factor 1, Glucose-sensitive Factor, PDX-1, IPF-1, IDX-1, STF-1, IUF-1, GSF, I

Cat. Num. AAA623485

• Gene Names: PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1
• Reactivity: Human
• Applications: ELISA, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PDX1; Polyclonal Antibody; phosphorylated (Thr11) (Pancreas/Duodenum Homeobox Protein 1; Insulin Promoter Factor 1; Islet/Duodenum Homeobox-1; Somatostatin-transactivating Factor 1; Insulin Upstream Factor 1; Glucose-sensitive Factor; PDX-1; IPF-1; IDX-1; STF-1; IUF-1; GSF; I; Anti -PDX1; anti-PDX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDX1 when phosphorylatedat Thr11.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PDX1 antibody: ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA and Dot Blot.; Dilution: ELISA: 1:1,000; Dot Blot: 1:500
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr11 of human PDX1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-IPF-pT11 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-PDX1 antibody

PDX1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in its gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).

Product Categories/Family for anti-PDX1 antibody

Antibodies; Abs to Transcription Factors; Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 4557673
• NCBI GeneID: 3651
• NCBI Accession #: NP_000200.1
• NCBI GenBank Nucleotide #: NM_000209.3
• UniProt Accession #: P52945; O60594; Q5VYW2
• Molecular Weight: 30,771 Da
• NCBI Official Full Name: pancreas/duodenum homeobox protein 1
• NCBI Official Synonym Full Names: pancreatic and duodenal homeobox 1
• NCBI Official Symbol: PDX1
• NCBI Official Synonym Symbols: GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1
• NCBI Protein Information: pancreas/duodenum homeobox protein 1; IPF-1; IUF-1; OTTHUMP00000018174; glucose-sensitive factor; insulin upstream factor 1; islet/duodenum homeobox-1; somatostatin transcription factor 1; pancreatic-duodenal homeobox factor 1; somatostatin-transactivating factor 1; insulin promoter factor 1, homeodomain transcription factor
• UniProt Protein Name: Pancreas/duodenum homeobox protein 1
• Protein Family: Pyridoxal 5'-phosphate synthase
• UniProt Gene Name: PDX1
• UniProt Synonym Gene Names: IPF1
• UniProt Entry Name: PDX1_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq]

Uniprot Description

PDX1: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP. Interacts with the methyltransferase SETD7. Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q12.1

Cellular Component: nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein heterodimerization activity; protein complex binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to nicotine; central nervous system development; response to glucocorticoid stimulus; exocrine pancreas development; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; response to chlorate; response to vitamin; negative regulation of cell proliferation; detection of glucose; morphogenesis of embryonic epithelium; positive regulation of cell proliferation; response to wounding; response to iron(II) ion; positive regulation of DNA binding; response to drug; generation of precursor metabolites and energy; organ regeneration; smoothened signaling pathway; organ senescence; glucose metabolic process; positive regulation of insulin secretion; stem cell differentiation; liver development; endocrine pancreas development; organ morphogenesis; response to leucine; gut development; insulin secretion; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; nitric oxide mediated signal transduction

Disease: Maturity-onset Diabetes Of The Young, Type 4; Diabetes Mellitus, Permanent Neonatal; Pancreatic Agenesis 1; Diabetes Mellitus, Noninsulin-dependent