DR6 Recombinant Protein | TNFRSF21 recombinant protein

DR6 Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123364

• Purity: >90% as determined by SDS-PAGE
• Synonyms: DR6; DR6 Protein; Rat; Recombinant (hFc Tag); Rat TNFRSF21/DR6 Protein (Fc Tag); tumor necrosis factor receptor superfamily; member 21; TNFRSF21 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His349

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 42
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat TNFRSF21 (NP_001101677.1) extracellular domain (Met 1-His 349) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TNFRSF21 recombinant protein

Background: TNFRSF21 (death receptor-6, DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N- and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain, and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production, suggested that DR6 serves as an important regulatory molecule in T-helper cell activation, and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs, heart, brain and pancreas. Some tumor cells overexpress DR6, typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion, which is subject to future investigations.

Product Categories/Family for TNFRSF21 recombinant protein

Death Receptor Signaling

References

Pan G, et al. (1998) Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett. 431(3): 351-6.Benschop R, et al. (2009) Tumor necrosis factor receptor superfamily member 21: TNFR-related death receptor-6, DR6. Adv Exp Med Biol. 647: 186-94.Klma M, et al. (2009) Functional analysis of the posttranslational modifications of the death receptor 6. Biochim Biophys Acta. 1793(10): 1579-87.

NCBI and Uniprot Product Information

• NCBI GI #: 157817151
• NCBI GeneID: 316256
• NCBI Accession #: NP_001101677.1
• NCBI GenBank Nucleotide #: NM_001108207.1
• UniProt Accession #: D3ZF92
• Molecular Weight: 71,870 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 21
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 21
• NCBI Official Symbol: Tnfrsf21
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 21
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 21
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf21
• UniProt Synonym Gene Names: Dr6
• UniProt Entry Name: TNR21_RAT

Uniprot Description

Promotes apoptosis, possibly via a pathway that involves the activation of NF-kappa-B. Can also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm. Plays a role in neuronal apoptosis, including apoptosis in response to amyloid peptides derived from APP, and is required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP). N-APP binds TNFRSF21; this triggers caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Plays a role in signaling cascades triggered by stimulation of T-cell receptors, in the adaptive immune response and in the regulation of T-cell differentiation and proliferation. Negatively regulates T-cell responses and the release of cytokines such as IL4, IL5, IL10, IL13 and IFNG by Th2 cells. Negatively regulates the production of IgG, IgM and IgM in response to antigens. May inhibit the activation of JNK in response to T-cell stimulation (). Negatively regulates oligodendrocyte survival, maturation and myelination.