Goat ARP2-3 Polyclonal Antibody | anti-ACTR3 antibody

ARP2-3 (Actin Related Protein 2/3 Complex, Subunit 1B, 41kD (ARP2/3 Subunit 1B, ARPC1B, ARC41, p40-ARC, p41-ARC, ARP2/3 Protein Complex Subunit p41)

Cat. Num. AAA623257

• Gene Names: ACTR3; ARP3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ARP2-3; Polyclonal Antibody; ARP2-3 (Actin Related Protein 2/3 Complex; Subunit 1B; 41kD (ARP2/3 Subunit 1B; ARPC1B; ARC41; p40-ARC; p41-ARC; ARP2/3 Protein Complex Subunit p41); Anti -ARP2-3 (Actin Related Protein 2/3 Complex; anti-ACTR3 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human ARP2-3 at ~37kD in human liver lysates (calculated MW of 40.9kD according to NP_005711). A minor band of unknown identity was also consistently observed at 28kDa.. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ACTR3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32,000; Western Blot: 2-4 ug/ml
• Immunogen: Synthetic peptide corresponding to human ARP2-3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ARP2-3 in Human Liver lysate (RIPA buffer, 35μg total protein per lane) using ARP2-3 antibody (2μg/ml).)

Related Product Information for anti-ACTR3 antibody

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

Product Categories/Family for anti-ACTR3 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5031573
• NCBI GeneID: 10096
• NCBI Accession #: NP_005712.1
• NCBI GenBank Nucleotide #: NM_005721.3
• UniProt Accession #: P61158; P32391; Q53QM2; Q59FV6
• Molecular Weight: 47,371 Da
• NCBI Official Full Name: actin-related protein 3
• NCBI Official Synonym Full Names: ARP3 actin-related protein 3 homolog (yeast)
• NCBI Official Symbol: ACTR3
• NCBI Official Synonym Symbols: ARP3
• NCBI Protein Information: actin-related protein 3; OTTHUMP00000203980; actin-like protein 3
• UniProt Protein Name: Actin-related protein 3
• UniProt Gene Name: ACTR3
• UniProt Synonym Gene Names: ARP3
• UniProt Entry Name: ARP3_HUMAN

NCBI Description

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. [provided by RefSeq]

Uniprot Description

Arp3: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Belongs to the actin family. ARP3 subfamily.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: Golgi membrane; Arp2/3 protein complex; focal adhesion; membrane; hemidesmosome; lamellipodium; excitatory synapse; actin filament; podosome; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton; ATP binding

Biological Process: positive regulation of filopodium formation; axon guidance; meiotic chromosome movement towards spindle pole; regulation of myosin II filament assembly or disassembly; establishment and/or maintenance of cell polarity; positive regulation of dendrite morphogenesis; cytokinesis after meiosis; response to carbohydrate stimulus; spindle localization; response to antibiotic; asymmetric cell division; ephrin receptor signaling pathway; innate immune response; cell motility