anti-Mouse Leptin Receptor Polyclonal Antibody | anti-LEPR antibody
Anti - Leptin Receptor (OBR, LEPR, B219, Obese Receptor)
Gene Names
LEPR; OBR; OB-R; CD295; LEP-R; DKFZp686B1731
Reactivity
Mouse
Applications
ELISA
Purity
Purified by immunoaffinity chromatography.
Synonyms
Leptin Receptor; Polyclonal Antibody; Anti - Leptin Receptor (OBR; LEPR; B219; Obese Receptor); Leptin Receptor (OBR; anti-LEPR antibody
Host
Host: Rabbit
Source: Mouse
Source: Mouse
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse sequence is unique to the OBRb long form. It is not found in OBR isoforms a and c-f. It is 94% conserved rat, 88% in monkey, human and 77% in porcine OBRb. Has no significant homology to gp130.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Sequence Length
1165
Applicable Applications for anti-LEPR antibody
ELISA
Application Notes
Suitable for use in ELISA. Other applications not tested.
Dilution: ELISA: 1:10,000-50,000 10-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: ELISA: 1:10,000-50,000 10-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
An 18 amino acid sequence near the C-terminus of mouse OBRb (long form) s (1).. The peptide was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-LEPR antibody
Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (146 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LEPR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894aa). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling. Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb. The absence of functional leptin in ob/ob and the long form OBRb in db/db mice due to abnormal splicing produces severe obesity. The nature of leptin resistance in other obesity models is not clear.
Product Categories/Family for anti-LEPR antibody
References
• Tartaglia, LA (1995) Cell 83, 1263-1271; Chen, H (1995) Cell 84, 491-495; Lee, G-H, et al (1996) Nature 379, 632-6352; Cioffi JA et al (1996) Nature Med. 2, 585-589; Takya K et al (1996) BBRC 225, 75-83; Considine,R.V et al (1996) Diabetes 45, 992-994; Considine,R.V et al (1997) BBRC 233, 248-252
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
132,494 Da
NCBI Official Full Name
leptin receptor
NCBI Official Synonym Full Names
leptin receptor
NCBI Official Symbol
LEPR
NCBI Official Synonym Symbols
OBR; OB-R; CD295; LEP-R; DKFZp686B1731
NCBI Protein Information
leptin receptor; huB219; OB receptor; OTTHUMP00000010692; OTTHUMP00000010693; OTTHUMP00000010694; OTTHUMP00000010695
UniProt Protein Name
Leptin receptor
Protein Family
UniProt Gene Name
LEPR
UniProt Synonym Gene Names
DB; OBR
UniProt Entry Name
LEPR_HUMAN
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Product Notes
The LEPR lepr (Catalog #AAA622800) is an Antibody produced from Host: Rabbit Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti - Leptin Receptor (OBR, LEPR, B219, Obese Receptor) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Leptin Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA. Suitable for use in ELISA. Other applications not tested. Dilution: ELISA: 1:10,000-50,000 10-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the LEPR lepr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leptin Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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