Western Blot (WB)
(Western Blot using MBS622333 shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70kD corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead).)
Chicken anti-Human LINE-1 Polyclonal Antibody | anti-L1TD1 antibody
LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2)
Gene Names
L1TD1; ECAT11; RP5-1155K23.3
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by selective precipitation and salt fractionation followed by extensive dialysis.
Synonyms
LINE-1; Polyclonal Antibody; LINE-1 (L1; Long Interspersed Element; Retrotransposon ORF2); Anti -LINE-1 (L1; anti-L1TD1 antibody
Host
Chicken
Reactivity
Human
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes human LINE-1 at ~149kD.
Purity/Purification
Purified
Purified by selective precipitation and salt fractionation followed by extensive dialysis.
Purified by selective precipitation and salt fractionation followed by extensive dialysis.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-L1TD1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:10,000
Western Blot: 1:2000-1:10,000
Dilution: ELISA: 1:10,000
Western Blot: 1:2000-1:10,000
Immunogen
Synthetic peptides corresponding to regions within the endonuclease domain of human LINE-1, conjugated to KLH.
Species Sequence Homology
Chimpanzee; 100%, macaque, fruit fly, bovine, canine, opossum, rat; 69-88%
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot using MBS622333 shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70kD corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead).)
Western Blot (WB)
(Western Blot using MBS622333 shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70kD corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead).)
Related Product Information for anti-L1TD1 antibody
The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.
Product Categories/Family for anti-L1TD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~149kD
NCBI Official Full Name
LINE-1 type transposase domain-containing protein 1
NCBI Official Synonym Full Names
LINE-1 type transposase domain containing 1
NCBI Official Symbol
L1TD1
NCBI Official Synonym Symbols
ECAT11; RP5-1155K23.3
NCBI Protein Information
LINE-1 type transposase domain-containing protein 1; ES cell-associated protein 11
UniProt Protein Name
LINE-1 type transposase domain-containing protein 1
Protein Family
UniProt Gene Name
L1TD1
UniProt Synonym Gene Names
ECAT11
UniProt Entry Name
LITD1_HUMAN
Uniprot Description
L1TD1: Belongs to the transposase 22 family.
Chromosomal Location of Human Ortholog: 1p31.3
Research Articles on L1TD1
Similar Products
Product Notes
The L1TD1 l1td1 (Catalog #AAA622333) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LINE-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:10,000 Western Blot: 1:2000-1:10,000. Researchers should empirically determine the suitability of the L1TD1 l1td1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LINE-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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