Goat anti-Human CRABP2 Polyclonal Antibody | anti-CRABP2 antibody

CRABP2 (CRABP-2, CRABPII, CRABP-II, Cellular Retinoic Acid-Binding Protein 2, RBP6, Retinoic Acid Binding Protein 6)

Cat. Num. AAA622202

• Gene Names: CRABP2; RBP6; CRABP-II
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CRABP2; Polyclonal Antibody; CRABP2 (CRABP-2; CRABPII; CRABP-II; Cellular Retinoic Acid-Binding Protein 2; RBP6; Retinoic Acid Binding Protein 6); Anti -CRABP2 (CRABP-2; anti-CRABP2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRABP2 at ~14kD.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

• Applicable Applications for anti-CRABP2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5ug/ml
• Immunogen: Recombinant human CRABP2 aa1-138; P29373 (E. coli).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of CRABP2. Lysates from human MCF-7, MDA-MB-468 and T47D cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.5ug/ml anti-CRABP2, as described in Protocols for Immunoblotting.)

Related Product Information for anti-CRABP2 antibody

CRABP2 (Cellular retinoic acid binding protein type II; also RABP2) is a 14kD cytosolic member of the fatty acid binding protein family of molecules. It is expressed by a variety of cell types, including keratinocytes, corneal epithelium, fibroblasts and visceral smooth muscle. CRABP2 binds bioactive retinoic acid and transports it to the nucleus, where, in contrast to CRABP1, it facilitates its transfer to the retinoic acid receptor. Human CRABP2 is 138aa in length and contains one NLS (aa21-31) and a series of beta-strands forming a beta-barrel that accommodates retinoic acid in its interior (aa41-135).

Product Categories/Family for anti-CRABP2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4503029
• NCBI GeneID: 1382
• NCBI Accession #: NP_001869.1
• NCBI GenBank Nucleotide #: NM_001878.3
• UniProt Accession #: P29373; Q6ICN6; B2R4Z8; D3DVC5; Q5SYZ4
• Molecular Weight: 15,693 Da
• NCBI Official Full Name: cellular retinoic acid-binding protein 2
• NCBI Official Synonym Full Names: cellular retinoic acid binding protein 2
• NCBI Official Symbol: CRABP2
• NCBI Official Synonym Symbols: RBP6; CRABP-II
• NCBI Protein Information: cellular retinoic acid-binding protein 2; OTTHUMP00000038730; OTTHUMP00000038732; cellular retinoic acid-binding protein II
• UniProt Protein Name: Cellular retinoic acid-binding protein 2
• Protein Family: Cellular retinoic acid-binding protein
• UniProt Gene Name: CRABP2
• UniProt Entry Name: RABP2_HUMAN

NCBI Description

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Uniprot Description

CRABP2: Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: retinoid binding; retinoic acid binding; transporter activity; retinol binding; retinal binding

Biological Process: embryonic forelimb morphogenesis; epidermis development; regulation of transcription, DNA-dependent; retinoic acid metabolic process; signal transduction; transmembrane transport