Immunohistochemistry (IHC)
(TRIM5 (2ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)
Rabbit anti-Human TRIM5 Polyclonal Antibody | anti-TRIM5 antibody
TRIM5 (Tripartite Motif Protein 5, EC 6.3.2.-, RNF88)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Immunohistochemistry: 2ug/ml
Western Blot: 1-2ug/ml
Immunohistochemistry (IHC)
(TRIM5 (2ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(TRIM5 (2ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
Uniprot Description
TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 11p15
Cellular Component: cytoplasm; cytosol
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; pattern recognition receptor activity; ligase activity
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of MAPKKK cascade; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of transcription factor activity; pattern recognition receptor signaling pathway; activation of innate immune response; defense response to virus; regulation of lipopolysaccharide-mediated signaling pathway; activation of NF-kappaB transcription factor
Research Articles on TRIM5
2. TRIM5 alpha recognises the multimerised capsid (viral core) of an incoming virus by its alpha-isoform specific SPRY domain and is believed to be involved in innate immunity to control retroviral infection.
3. These results suggest that Hsp70 plays critical roles in the cellular management against the TRIM5alpha-induced cellular insults.
4. Results describe the action of a human HIV-1 inhibitor modeled after AoT5Cyp, constructued by fusing human CypA to human TRIM5.
5. Data show significant associations between SNPs in RARA, RARB, TOP2B and RARG, RXRA, TLR3, TRIM5 and RIG-I genes and rubella virus-specific cytokine immune responses.
6. increased representation of the minor allele of the 5'UTR SNP (rs3824949) in the antiretroviral TRIM5(tripartite motif protein5) gene was associated with an allele dose-related increase in rubella antibody.
7. A rare TRIM5 allele from Baka pygmies encodes a truncated TRIM5alpha protein defective for retrovirus restriction.
8. High expression of TRIM5alphahu is associated with reduced susceptibility to HIV-1 infection, but does not contribute to the control of primary HIV-1 viremia.
9. These results indicate that, in addition to CA-specific binding, the PRYSPRY domain possesses a second function, possibly binding of a cofactor, that is essential for retroviral restriction activity by TRIM5alpha.
10. The TRIM5alpha B-box 2 domain promotes cooperative binding to the retroviral capsid by mediating higher-order self-association.
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Product Notes
The TRIM5 trim5 (Catalog #AAA621513) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM5 (Tripartite Motif Protein 5, EC 6.3.2.-, RNF88) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry: 2ug/ml Western Blot: 1-2ug/ml. Researchers should empirically determine the suitability of the TRIM5 trim5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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