Goat anti-Mouse Carbohydrate Sulfotransferase 5 Polyclonal Antibody | anti-CHST5 antibody

Carbohydrate Sulfotransferase 5 (CHST5, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5, ALYE870, FLJ22167, GlcNAc6ST-3, GST4-alpha, hIGn6ST, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, MGC74625, PRO1886)

Cat. Num. AAA621397

• Gene Names: CHST5; gn6st-3; hIGn6ST; MGC74625; I-GlcNAc6ST; glcNAc6ST-3; I-GlcNAc-6-ST
• Reactivity: Mouse
• Purity: Affinity Purified; Purified by Immunoaffinity chromatography.
• Synonyms: Carbohydrate Sulfotransferase 5; Polyclonal Antibody; Carbohydrate Sulfotransferase 5 (CHST5; Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5; ALYE870; FLJ22167; GlcNAc6ST-3; GST4-alpha; hIGn6ST; I-GlcNAc6ST; Intestinal GlcNAc-6-sulfotransferase; MGC74625; PRO1886); Anti -Carbohydrate Sulfotransferase 5 (CHST5; anti-CHST5 antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Carbohydrate Sulfotransferase 5. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Application Notes: Suitable for use in Peptide ELISA.; Dilution: Peptide ELISA: 1:32,000
• Immunogen: Synthetic peptide corresponding to C-DSFKWASSTEKQPE from the C Terminus of mouse Carbohydrate Sulfotransferase 5 (NP_064334.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CHST5 antibody

CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha) is likely a 53-56kD member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position #6 of mucin-associated GlcNAc. Human CHST5 is a 390aa type II transmembrane protein. It contains a short cytoplasmic segment (aa1-9) and a long luminal domain (aa28-390) that contains an enzymatic region (aa180-358). There is one alternate start site that is 22aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa28-390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4- beta. Over aa 28-390, human GST4-alpha/CHST5 is 78% aa identical to mouse GST4.

Product Categories/Family for anti-CHST5 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 21362052
• NCBI GeneID: 23563
• NCBI Accession #: NP_078809.2
• NCBI GenBank Nucleotide #: NM_024533.3
• UniProt Accession #: Q9GZS9; Q7LCN3; Q9UBY3; B2RV23
• Molecular Weight: 46,161 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 5
• NCBI Official Synonym Full Names: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
• NCBI Official Symbol: CHST5
• NCBI Official Synonym Symbols: gn6st-3; hIGn6ST; MGC74625; I-GlcNAc6ST; glcNAc6ST-3; I-GlcNAc-6-ST
• NCBI Protein Information: carbohydrate sulfotransferase 5; GST4-alpha; OTTHUMP00000174950; intestinal GlcNAc-6-sulfotransferase; N-acetylglucosamine 6-O-sulfotransferase 3; intestinal N-acetylglucosamine-6-O-sulfotransferase; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha
• UniProt Protein Name: Carbohydrate sulfotransferase 5
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST5
• UniProt Entry Name: CHST5_HUMAN

NCBI Description

The carbohydrates of glycoconjugates are highly diverse structures with variation in monosaccharide composition, glycosidic linkage positions, and branching of chains. Further diversity is added by the covalent addition of sulfate moieties to particular hydroxyl groups and amino groups of saccharides. The sulfate modifications of glycoproteins can be extensive in amount and frequently occur at high density. They can have a profound effect on the physiochemical properties of the glycoconjugates, at least in part through the addition of negative charge. Carbohydrate sulfation plays a critical role in many biologic processes. CHST5 belongs to the GST family of sulfotransferases, which also includes CHST1 (MIM 603797), CHST2 (MIM 603798), CHST3 (MIM 603799), and LSST. These enzymes are 6-O-sulfotransferases, which add sulfate to C6 of galactose (Gal), N-acetylgalactosamine (GalNAc), or N-acetylglucosamine (GlcNAc) (Lee et al., 1999 [PubMed 10491328]).[supplied by OMIM]

Uniprot Description

CHST5: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; EC 2.8.2.-

Chromosomal Location of Human Ortholog: 16q22.3

Cellular Component: Golgi membrane; Golgi apparatus; intrinsic to Golgi membrane; integral to membrane

Molecular Function: sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; protein amino acid sulfation; keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; pathogenesis