Rabbit Hepatocyte nuclear factor 4-alpha Polyclonal Antibody | anti-HNF4A antibody

Anti-HNF-4-alpha Antibody

Cat. Num. AAA177518

• Gene Names: HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• Reactivity: Human, Mouse, Rat. No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Hepatocyte nuclear factor 4-alpha; Polyclonal Antibody; Anti-HNF-4-alpha Antibody; FLJ39654 antibody; Hepatic nuclear factor 4 alpha antibody; Hepatocyte nuclear factor 4 alpha antibody; Hepatocyte nuclear factor 4 antibody; Hepatocyte nuclear factor 4-alpha antibody; HNF 4 alpha antibody; HNF 4 antibody; HNF-4-alpha antibody; HNF4 antibody; HNF4A antibody; HNF4A_HUMAN antibody; HNF4a7 antibody; HNF4a8 antibody; HNF4a9 antibody; Hnf4alpha antibody; HNF4alpha10/11/12 antibody; MODY 1 antibody; MODY antibody; MODY1 antibody; NR2A1 antibody; NR2A21 antibody; Nuclear receptor subfamily 2 group A member 1 antibody; OTTHUMP00000031060 antibody; OTTHUMP00000031062 antibody; TCF 14 antibody; TCF antibody; TCF-14 antibody; TCF14 antibody; Tcf4 antibody; Transcription factor 14; hepatic nuclear factor antibody; Transcription factor 14 antibody; Transcription factor HNF 4 antibody; Transcription factor HNF-4 antibody; Transcription factor HNF4 antibody; hepatocyte nuclear factor 4; alpha; anti-HNF4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat. No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 474

• Applicable Applications for anti-HNF4A antibody: Western Blot (WB), Immunohistochemistry (IHC-P)
• Application Notes: Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Rat; Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml; Tested Species: Hu, Ms, Rat; Antigen Retrieval: By Heat; Immunohistochemistry (Frozen Section): Concentration: 0.5-1ug/ml; Tested Species: Ms, Rat.; WB: The detection limit for HNF-4-alpha is approximately 0.25ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequences identity with mouse and rat HNF-4-alpha, respectively.
• Relevant Detection Systems: We provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit (MBS176460) in WB, supported by MBS176451 in IHC(P) and IHC(F).
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- HNF-4-alpha antibody, MBS177518, Western blottingAll lanes: Anti HNF-4-alpha (MBS177518) at 0.5ug/mlWB: Recombinant Human HNF-4-alpha Protein 0.5ngPredicted bind size: 38KDObserved bind size: 38KD)

Western Blot (WB)

(Anti- HNF-4-alpha antibody, MBS177518, Western blottingAll lanes: Anti HNF-4-alpha (MBS177518) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: RH35 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD )

Immunohistochemistry (IHC)

(Anti- HNF-4-alpha antibody, MBS177518, IHC(P)IHC(P): Rat Kidney Tissue)

Immunohistochemistry (IHC)

(Anti- HNF-4-alpha antibody, MBS177518, IHC(P)IHC(P): Mouse Kidney Tissue )

Immunohistochemistry (IHC)

(Anti- HNF-4-alpha antibody, MBS177518, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti- HNF-4-alpha antibody, MBS177518, IHC(F)IHC(F): Mouse Liver Tissue)

Immunohistochemistry (IHC)

(Anti- HNF-4-alpha antibody, MBS177518, IHC(F)IHC(F): Rat Kindey Tissue)

Related Product Information for anti-HNF4A antibody

Background: Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

NCBI and Uniprot Product Information

• NCBI GI #: 31077205
• NCBI GeneID: 3172
• NCBI Accession #: NP_000448.3
• NCBI GenBank Nucleotide #: NM_000457.4
• UniProt Accession #: P41235; O00659; O00723; Q14540; Q5QPB8; Q6B4V5; Q6B4V6; Q6B4V7; Q92653; Q92654; A5JW41; B2RPP8
• Molecular Weight: 43,988 Da
• NCBI Official Full Name: hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
• NCBI Official Synonym Full Names: hepatocyte nuclear factor 4, alpha
• NCBI Official Symbol: HNF4A
• NCBI Official Synonym Symbols: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• NCBI Protein Information: hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1
• UniProt Protein Name: Hepatocyte nuclear factor 4-alpha
• Protein Family: Hepatocyte nuclear factor
• UniProt Gene Name: HNF4A
• UniProt Synonym Gene Names: HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
• UniProt Entry Name: HNF4A_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; transcription factor activity; fatty acid binding; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; xenobiotic metabolic process; regulation of gastrulation; response to glucose stimulus; ornithine metabolic process; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis

Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent