Western Blot (WB)
(Western Blot Analysis: HeLa cell lysate was resolved by SDS-PAGE, transferred to PVDF and probed with MBS621235 (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G3BP1 (~68kD).)
Rabbit G3BP-1 Polyclonal Antibody
G3BP-1 (GTPase activating protein (SH3 domain) binding protein 1, ATP-dependent DNA helicase VIII, G3BP, G3BP-1, GAP SH3 domain-binding protein 1, HDH-VIII, MGC111040, Ras GTPase-activating protein-binding protein 1)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
SerumSerum.
Synonyms
G3BP-1; Polyclonal Antibody; G3BP-1 (GTPase activating protein (SH3 domain) binding protein 1; ATP-dependent DNA helicase VIII; G3BP; GAP SH3 domain-binding protein 1; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1); Anti -G3BP-1 (GTPase activating protein (SH3 domain) binding protein 1; anti-G3BP-1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human G3BP1 at its C-terminal region. Species Crossreactivity
Purity/Purification
Serum
Serum.
Serum.
Form/Format
Supplied as a liquid, 0.05% NaN3.
Applicable Applications for anti-G3BP-1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic peptide corresponding to C-terminal region of human G3BP1. Cellular Localization: Cell membrane › sarcolemma; Peripheral membrane protein; Cytoplasmic side
Positive Control
HeLa cell lysates.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot Analysis: HeLa cell lysate was resolved by SDS-PAGE, transferred to PVDF and probed with MBS621235 (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G3BP1 (~68kD).)
Western Blot (WB)
(Western Blot Analysis: HeLa cell lysate was resolved by SDS-PAGE, transferred to PVDF and probed with MBS621235 (1:1,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G3BP1 (~68kD).)
Related Product Information for anti-G3BP-1 antibody
G3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs). It was first discovered by its binding to RasGAP. G3BPs have been shown to be involved in a number of mitogenic signaling pathways. G3BP1 is also known to be over expressed in many human cancers (Kim, 2007) and its expression appears to be inversely correlated with PTEN expression (Kim, 2007). Interestingly, G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007). Furthermore, the G3BPs have two traditional RNA binding motifs.
Product Categories/Family for anti-G3BP-1 antibody
Similar Products
Product Notes
The G3BP-1 (Catalog #AAA621235) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The G3BP-1 (GTPase activating protein (SH3 domain) binding protein 1, ATP-dependent DNA helicase VIII, G3BP, G3BP-1, GAP SH3 domain-binding protein 1, HDH-VIII, MGC111040, Ras GTPase-activating protein-binding protein 1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's G3BP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the G3BP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G3BP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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