Rabbit anti-Human Glutamic Acid Decarboxylase Polyclonal Antibody
Anti - Glutamic Acid Decarboxylase (GAD)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
Glutamic Acid Decarboxylase; Polyclonal Antibody; Anti - Glutamic Acid Decarboxylase (GAD); Glutamic Acid Decarboxylase (GAD); anti-Glutamic Acid Decarboxylase antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognize both 65 and 67kD bands of human GAD.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Concentration
~1mg/ml (varies by lot)
Sequence Length
180
Applicable Applications for anti-Glutamic Acid Decarboxylase antibody
ELISA, Western Blot
Application Notes
Western Blot: 1-10ug/ml using Chemiluminescence technique.
ELISA: 1:10,000-1:100,000; using 50-100ng control peptide/well.
ELISA: 1:10,000-1:100,000; using 50-100ng control peptide/well.
Immunogen
16aa peptide from GAD65/67 of human, conjugated to KLH. Location
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Glutamic Acid Decarboxylase antibody
g-Aminobutyric acid (GABA) is the major known inhibitory neurotransmitter. The rate-limiting step in the synthesis of GABA is the decarboxylation of glutamate by glutamate decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.115). In the CNS GAB is entirely restricted to GABAergic neurons. GAD is also present in the b-cells of the pancreas and autoantibodies to various GAD polypeptides are detected in insulin-dependent diabetes mellitus. Cloning of GAD genes have identified two subtypes: GAD65 (65kD; human 585 AA chromosome 10) and GAD67 (67kD; human 594 AA, chromosome 2) share approx. 65% amino acid homology. The N-terminus is the most divergent while the C-terminus is highly conserved. Although both GAD isoforms catalyzes the conversion of GABA but interact differently with the co-factor pyridoxal 5\'-phosphate suggesting their activities are differentially regulated. GAD67 is cytosolic, while GAD65 is membrane associated. GAD65 is a major autoantigen in diabetes mellitus and stiff-man syndrome, a rare disease of the brain.
Product Categories/Family for anti-Glutamic Acid Decarboxylase antibody
References
• 1. Wyborksi RJ et al (1990) Mol. Brain,. Res. 8, 193-198; Julien JF et al (1990) J Neurochem. 54, 703; Katarove Z et al (1990) Ewur. J. Neurosci. 2, 190; Michelson BK et al (1992) Diabetes 41, 1182; Faulkner-Jones BE (1993) Endocrinol. 133, 2962;
• 2. Dirkxx R et al (1995) JBC 270, 2241; Solimena M (unpublihsed results on file).
• 2. Dirkxx R et al (1995) JBC 270, 2241; Solimena M (unpublihsed results on file).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
glutamic acid decarboxylase
Similar Products
Product Notes
The Glutamic Acid Decarboxylase (Catalog #AAA620847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - Glutamic Acid Decarboxylase (GAD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamic Acid Decarboxylase can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Western Blot: 1-10ug/ml using Chemiluminescence technique. ELISA: 1:10,000-1:100,000; using 50-100ng control peptide/well. Researchers should empirically determine the suitability of the Glutamic Acid Decarboxylase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamic Acid Decarboxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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