Mouse anti-Human CD11a Monoclonal Antibody | anti-CD11a antibody
CD11a (Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide, Integrin alpha-L, ITGAL, Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain, LFA 1 alpha (LFA1A), Ly15, Ly21, Lymphocyte function associated antigen 1, p180) (BSA & Az
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
CD11a; Monoclonal Antibody; CD11a (Antigen CD11A (p180); lymphocyte function associated antigen 1; alpha polypeptide; Integrin alpha-L; ITGAL; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain; LFA 1 alpha (LFA1A); Ly15; Ly21; Lymphocyte function associated antigen 1; p180) (BSA & Az; Antigen CD11A (p180); p180; anti-CD11a antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
CRIS-3
Specificity
Recognizes human CD11a.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-CD11a antibody
FLISA, Immunohistochemistry (IHC) Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
FC/FACS: 0.5-1ug/million cells
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
Immunogen
Stimulated human leukocytes. Cellular Localization: Cell surface
Conjugate
MaxLight490
Positive Control
JY, HUT-78, MOLT-4, HL60, U937, and human leukocytes. Human lymph nodes and tonsils.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Categories/Family for anti-CD11a antibody
References
1. Reinherz EL et al. eds. Leukocyte Typing II, Oxford University Press, Oxford, 1985 2. Knapp W. et al. Leukocyte Typing IV, Oxford Unioversity Press, Oxford, 1989. 3. Petruzzelli L et al. Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18. J Immunol 1995, 155(2):854-866. 4. Edwards CP et al. Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.J Biol Chem 1998, 273(44):28937-44. 5. Shang XZ and Issekutz AC. Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol 1998, 28(6):1970-1979 6. Tian L et al. The neuronal glycoprotein telencephalin is a cellular ligand for the CD11a/CD18 leukocyte integrin. J Immunol 1997, 158(2):928-936.
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Product Notes
The CD11a (Catalog #AAA6204371) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD11a (Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide, Integrin alpha-L, ITGAL, Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain, LFA 1 alpha (LFA1A), Ly15, Ly21, Lymphocyte function associated antigen 1, p180) (BSA & Az reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11a can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunohistochemistry (IHC) Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF). FC/FACS: 0.5-1ug/million cells IP: 0.5-1ug/500ug protein lysate IF: 0.5-1ug/ml IHC-F: 0.5-1.0ug/ml for 30 minutes at RT Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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