Rabbit anti-Rat Connexin 33 Polyclonal Antibody | anti-GJB6 antibody

Connexin 33 (CX33, Gap Junction Alpha 7 Protein, CXA-7)

Cat. Num. AAA618853

• Gene Names: GJB6; ED2; EDH; HED; CX30; HED2; DFNA3; ECTD2; DFNA3B; DFNB1B
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Connexin 33; Polyclonal Antibody; Connexin 33 (CX33; Gap Junction Alpha 7 Protein; CXA-7); Anti -Connexin 33 (CX33; anti-GJB6 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat CX33. Immunogenic peptide sequence is specific for CX33 and no significant homology is seen with other Connexins. Sequence homology of rat CX33 peptide with other species is not known.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of 40% glycerol.

• Applicable Applications for anti-GJB6 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using Chemiluminescence technique.; ELISA: 1:10,000-100,000 using 50-100ng control peptide per well. See C7854-27B.
• Immunogen: A 17 amino acid peptide sequence within the C-terminal cytoplasmic domain of Rat CX33
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GJB6 antibody

Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Daltons. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junction may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, CX26-50. Hydropathy analyses of CX sequences predicts 4 transmembrane domains, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.

Product Categories/Family for anti-GJB6 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 55925665
• NCBI GeneID: 10804
• UniProt Accession #: O95452; Q5Q1H9; Q5Q1I0; Q5Q1I1; Q5T5U0; Q8IUP0; B3KQN2
• Molecular Weight: 30,387 Da
• NCBI Official Full Name: connexin 30
• NCBI Official Synonym Full Names: gap junction protein, beta 6, 30kDa
• NCBI Official Symbol: GJB6
• NCBI Official Synonym Symbols: ED2; EDH; HED; CX30; HED2; DFNA3; ECTD2; DFNA3B; DFNB1B
• NCBI Protein Information: gap junction beta-6 protein; connexin 30; connexin-30; gap junction protein, beta 6 (connexin 30); ectodermal dysplasia 2, hidrotic (Clouston syndrome)
• UniProt Protein Name: Gap junction beta-6 protein
• Protein Family: Gap junction beta-6 protein
• UniProt Gene Name: GJB6
• UniProt Synonym Gene Names: Cx30
• UniProt Entry Name: CXB6_HUMAN

NCBI Description

Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form (alpha or beta) of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

GJB6: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Beta-type (group I) subfamily. A connexon is composed of a hexamer of connexins. Interacts with CNST

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: connexon complex; apical plasma membrane; integral to membrane; cytosol

Biological Process: negative regulation of cell proliferation; sensory perception of sound; apoptosis; cell communication; response to lipopolysaccharide; ear morphogenesis; response to electrical stimulus; inner ear development; aging

Disease: Deafness, Autosomal Recessive 1a; Clouston Syndrome; Deafness, Autosomal Recessive 1b; Deafness, X-linked 2; Deafness, Autosomal Dominant 3b